【正文】 CGGATG AGAAGAGCTT 50 GCTCTTCGAT TCAGCGGCGG ACGGGTGAGT AATGCCTAGG AATCTGCCTG 100 GTAGTGGGGG ACAACGTTTC GAAAGGAACG CTAATACCGC ATACGTCCTA 150 CGGGAGAAAG CAGGGGACCT TCGGGCCTTG CGCTATCAGA TGAGCCTAGG 200 TCGGATTAGC TAGTTGGTGA GGTAATGGCT CACCAAGGCG ACGATCCGTA 250 ACTGGTCTGA GAGGATGATC AGTCACACTG GAACTGAGAC ACGGTCCAGA 300 CTCCTACGGG AGGCAGCAGT GGGGAATATT GGACAATGGG CGAAAGCCTG 350 ATCCAGCCAT GCCGCGTGTG TGAAGAAGGT CTTCGGATTG TAAAGCACTT 400 TAAGTTGGGA GGAAGGGCAT TAACCTAATA CGTTAGTGTT TTGACGTTAC 450 CGACAGAATA AGCACCGGCT AACTCTGTGC CAGCAGCCGC GGTAATACAG 500 AGGGTGCAAG CGTTAATCGG AATTACTGGG CGTAAAGCGC GCGTAGGTGG 550 TTTGTTAAGT TGGATGTGAA AGCCCCGGGC TCAACCTGGG AACTGCATCC 600 AAAACTGGCA AGCTAGAGTA CGGTAGAGGG TGGTGGAATT TCCTGTGTAG 650 CGGTGAAATG CGTAGATATA GGAAGGAACA CCAGTGGCGA AGGCGACCAC 700 CTGGACTGAT ACTGACACTG AGGTGCGAAA GCGTGGGGAG CAAACAGGAT 750 TAGATACCCT GGTAGTCCAC GCCGTAAACG ATGTCAACTA GCCGTTGGAA 800 TCCTTGAGAT TTTAGTGGCG CAGCTAACGC ATTAAGTTGA CCGCCTGGGG 850 AGTACGGCCG CAAGGTTAAA ACTCAAATGA ATTGACGGGG GCCCGCACAA 900 GCGGTGGAGC ATGTGGTTTA ATTCGAAGCA ACGCGAAGAA CCTTACCAGG 950 CCTTGACATG CAGAGAACTT TCCAGAGATG GATTGGTGCC TTCGGGAACT 1000 CTGACACAGG TGCTGCATGG CTGTCGTCAG CTCGTGTCGT GAGATGTTGG 1050 GTTAAGTCCC GTAACGAGCG CAACCCTTGT CCTTAGTTAC CAGCACGTTA 1100 TGGTGGGCAC TCTAAGGAGA CTGCCGGTGA CAAACCGGAG GAAGGTGGGG 1150 ATGACGTCAA GTCATCATGG CCCTTACGGC CTGGGCTACA CACGTGCTAC 1200 AATGGTCGGT ACAGAGGGTT GCCAAGCCGC GAGGTGGAGC TAATCTCACA 1250 AAACCGATCG TAGTCCGGAT CGCAGTCTGC AACTCGACTG CGTGAAGTCG 1300 GAATCGCTAG TAATCGCGAA TCAGAATGTC GCGGTGAATA CGTTCCCGGG 1350 CCTTGTACAC ACCGCCCGTC ACACCATGGG AGTGGGTTGCA CCAGAAGTAG 1400 CTAGTCTAAC CTTCGGGAGG ACGGTTACCA CGGTGTGATT CATGACTGGG 1450 常熟理工學(xué)院畢業(yè)設(shè)計(jì)（論文） 13 結(jié)果： 根據(jù)以上各個(gè)結(jié)果，得出： S1 菌株為假單胞菌屬 菌株 S2 的 16S rDNA 序列分析 表 菌株 S1 的鑒定結(jié)果 Accession Description Max score Total score Query coverage E value Max ident Uncultured bacterium clone N167 16S ribosomal RNA gene, partial sequence Pseudomonas sp. BSw10041N 16S ribosomal RNA, partial sequence 2680 2680 100% 100% gb|| Pseudomonas sp. PG12 16S ribosomal RNA gene, partial sequence 2680 2680 100% 100% Pseudomonas sp. WMQ7 16S ribosomal RNA gene, partial sequence 2680 2680 100% 100% Uncultured bacterium clone MP1040916b40 16S ribosomal RNA gene, partial sequence 2680 2680 100% 100% Uncultured Pseudomonas sp. clone SQ9_Pitesti 16S ribosomal RNA gene, partial sequence 2680 2680 100% 100% Pseudomonas putida gene for 16S rRNA, partial sequence, strain: KF715 2680 2680 100% 100% Pseudomonas putida gene for 16S rRNA, partial sequence, strain: NBRC 14164 2676 2676 100% 99% Pseudomonas sp. OCR2 gene for 16S rRNA, partial sequence 2676 2676 100% 99% Pseudomonas putida strain ATCC 12633 16S ribosomal RNA gene, partial sequence 2676 2676 100% 99% Uncultured bacterium clone N07 16S ribosomal RNA gene, partial sequence 2675 2675 100% 99% Uncultured Pseudomonas sp. clone AV_5NG03 16S ribosomal RNA gene, partial sequence 2675 2675 100% 99% Pseudomonas sp. WSCIII 16S ribosomal RNA gene, partial sequence 2675 2675 100% 99% Pseudomonas putida strain ATCC 11172 16S ribosomal RNA gene, partial sequence 2675 2675 100% 99% Uncultured bacterium clone 1H3C_22 16S ribosomal RNA gene, partial sequence 2669 2669 100% 99% Pseudomonas sp. CSNA6 16S ribosomal RNA gene, partial sequence 2669 2669 100% 99% Uncultured bacterium clone N163 16S ribosomal RNA gene, partial sequence 2669 2669 100% 99% 表 菌株 S2 的 16S rDNA 序列 常熟理工學(xué)院畢業(yè)設(shè)計(jì)（論文） 14 ACGCTGGCGG CAGGCCTAAC ACATGCAAGT CGAGCGGTAG AGAGAAGCTT 50 GCTTCTCTTG AGAGCGGCGG ACGGGTGAGT AATGCCTAGG AATCTGCCTG 100 GTAGTGGGGG ATAACGTTCG GAAACGGACG CTAATACCGC ATACGTCCTA 150 CGGGAGAAAG CAGGGGACCT TCGGGCCTTG CGCTATCAGA TGAGCCTAGG 200 TCGGATTAGC TAGTTGGTGG GGTAATGGCT CACCAAGGCG ACGATCCGTA 250 ACTGGTCTGA GAGGATGATC AGTCACACTG GAACTGAGAC ACGGTCCAGA 300 CTCCTACGGG AGGCAGCAGT GGGGAATATT GGACAATGGG CGAAAGCCTG 350 ATCCAGCCAT GCCGCGTGTG TGAAGAAGGT CTTCGGATTG TAAAGCACTT 400 TAAGTTGGGA GGAAGGGTTG TAGATTAATA CTCTGCAATT TTGACGTTAC 450 CGACAGAATA AGCACCGGCT AACTCTGTGC CAGCAGCCGC GGTAATACAG 500 AGGGTGCAAG CGTTAATCGG AATTACTGGG CGTAAAGCGC GCGTAGGTGG 550 TTTGTTAAGT TGGATGTGAA ATCCCCGGGC TCAACCTGGG AACTGCATTC 600 AAAACTGACT GACTAGAGTA TGGTAGAGGG TGGTGGAATT TCCTGTGTAG 650 CGGTGAAATG CGTAGATATA GGAAGGAACA CCAGTGGCGA AGGCGACCAC 700 CTGGACTAAT ACTGACACTG AGGTGCGAAA GCGTGGGGAG CAAACAGGAT 750 TAGATACCCT GGTAGTCCAC GCCGTAAACG ATGTCAACTA GCCGTTGGAA 800 GCCTTGAGCT TTTAGTGGCG CAGCTAACGC ATTAAGTTGA CCGCCTGGGG 850 AGTACGGCCG CAAGGTTAAA ACTCAAATGA ATTGACGGGG GCCCGCACAA 900 GCGGTGGAGC ATGTGGTTTA ATTCGAAGCA ACGCGAAGAA CCTTACCAGG 950 CCTTGACATC CAATGAACTT TCCAGAGATG GATTGGTGCC TTCGGGAACA 1000 TTGAGACAGG TGCTGCATGG CTGTCGTCAG CTCGTGTCGT GAGATGTTGG 1050 GTTAAGTCCC GTAACGAGCG CAACCCTTGT CCTTAGTTAC CAGCACGTAA 1100 TGGTGGGCAC TCTAAGGAGA CTGCCGGTGA CAAACCGGAG GAAGGTGGGG 1150 ATGACGTCAA GTCATCATGG CCCTTACGGC CTGGGCTACA CACGTGCTAC 1200 AATGGTCGGT ACAGAGGGTT GCCAAGCCGC GAGGTGGAGC TAATCCCATA 1250 AAACCGATCG TAGTCCGGAT CGCAGTCTGC AACTCGACTG CGTGAAGTCG 1300 GAATCGCTAG TAATCGCGAA TCAGAATGTC GCGGTGAATA CGTTCCCGGG 1350 CCTTGTACAC ACCGCCCGTC ACACCATGGG AGTGGGTTGC ACCAGAAGTA 1400 GCTAGTCTAA CCTTCGGGAG GACG