【正文】 per. Mycelium ball in the bottle, drying to constant heavy in the electric thermostat dry box, calculate its dry heavy with Precision electronic balance. Using ultraviolet Spectrophotometric method determinates of selenium content of mycelium. The excel mapping and SPSS statistical analysis will be used in the processing of experimental data. Results: When selenium concentration of culture is 20~35 μ g/l, the fastest growth rate and selenium status of the most powerful of Flammnlina velutipes 201 and white Flammnlina velutipes and mutation strain will be achieved in the culture. Conclusions: Comparing with the plate culture, mycelium growth rate and capacity of Seaccumulating reaches new standard in the liquid fermentation. Key words: Flammnlina velutipes； Selenium； ultraviolet radiation mutagenesis； Se accumulating plate culture； Se accumulating Liquid training 富硒金針菇菌株的選育畢業(yè)論文 III 目 錄 1 引言 .............................................................................................................................................. 1 金針菇的特性 ...................................................................................................................... 1 硒的生理生化功能 ............................................................................................................... 1 人體所適宜的膳食硒濃度 .................................................................................................... 1 2 材料和方法 .................................................................................................................................... 2 材料 .................................................................................................................................... 2 菌種 ......................................................................................................................... 2 藥品 ......................................................................................................................... 2 培養(yǎng)基 ...................................................................................................................... 3 儀器及設(shè)備 ................................................................................................................ 3 耗材 .......................................................................................................................... 3 方法 .................................................................................................................................... 3 一級菌種培養(yǎng)基的配置方法 ...................................................................................... 3 菌絲體轉(zhuǎn)管 ............................................................................................................... 5 紫外誘變 .................................................................................................................. 5 菌絲體的活化與復(fù)壯 ................................................................................................. 6 菌種保藏 ................................................................................................................... 6 固化富硒培養(yǎng) ............................................................................................................ 6 液體富硒培養(yǎng) ............................................................................................................ 7 固化金針菇菌絲的形態(tài)學(xué)檢測 .................................................................................... 7 液體培養(yǎng)金針菇菌絲球的形態(tài)學(xué)檢測 ........................................................................... 8 標(biāo)準(zhǔn)曲線的制作 ...................................................................................................... 9 3 結(jié)果與分析（討論） .....................................................................................................................10 紫外誘變對金針菇形態(tài)學(xué)特征的影響 ..................................................................................10 金針菇在液體富硒培養(yǎng)中的特性 .........................................................................................10 金針菇在液體富硒培養(yǎng)基中的生長特性 ...................................................................... 11 金針菇在液體富硒培養(yǎng)基中的富硒特性 ......................................................................13 對白色金針菇和金針菇 201 及其誘變菌種的相關(guān)參數(shù)的統(tǒng)計(jì)分析 .......................................29 紫外誘變對白色金針菇和金針菇 201 的生長速率以及富硒能力的影響 ........................29 白色金針菇和金針菇 201 的生長速率以及富硒能力的 獨(dú)立樣本 t 檢驗(yàn) .........................30 固體、液體培養(yǎng) 對金針菇生長速率和富硒能力的獨(dú)立樣本 t 檢驗(yàn) ...............................30 4 結(jié)論與展望 ...................................................................................................................................31 結(jié)論 ...................................................................................................................................31 展望 ...................................................................................................................................31 參考文獻(xiàn) ..........................................................................................................................................32 附錄 .................................................................................................................................................33 培養(yǎng)基配置的原則 .....................................................................................................................33 pH 值標(biāo)準(zhǔn)溶液配置說明 ............................................................................................................33 高壓蒸汽滅菌的方法步驟 ..........................................................................................................34 一級菌種轉(zhuǎn)管的注意事項(xiàng) ..........................................................................................................34 致 謝 ..........................................................................................................................................35 富硒金針菇菌株的選育畢業(yè)論文 1 富硒金針菇菌種的選育 1 引言 金針菇的