科大高分子課件四聚合物在生物高分子分離中的應(yīng)用-展示頁(yè)
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【正文】 100246μeof (108m2V1s1)pH Bare ca p il lary HEC HECg P4V P0 2 4 6 8 10M igr atio n t ime ( m in)321pH=a0 2 4 6 8 10M igration time (min)12pH=3b0 5 10 15 20 25Migration time (mi n)123pH =0 5 10 15 20 25Mi grati on ti me (mi n)123pH =dFormation of the HECgP4VP Electroosmotic flow as a function of pH. Comparison between a bare fusedsilica capillary, HEC and HECgP4VP coated capillary Electropherograms of a mixture of standard proteins. Separations were carried out using catHECgP4VP coated capillary. 1 = Lysozyme。 3 = Ribonuclease A. Electropherograms of a mixture of standard proteins at pH . 0 2 4 6 8 10 12M igration time (min)PH=first runfiftieth run(a)1230 2 4 6 8 10 12 14M igration time ( min)first runfiftieth runpH=(b) 1230 2 4 6 8M igration time (min)first runfiftieth runpH=(c)123Protein Bare capillary N (plate/m) b HEC N (plate/m) RSD (%) CatHEC2 N(plate/m) RSD (%) Cytochrome C 9300 104000 186000 Lysozyme 162022 265000 Ribonuclease A 17300 180000 282022 Electrophoresis, 2022, 29, 14601466. Electropherograms of a mixture of standard proteins in different pH. Separations were carried out using catHEC2 coated capillary 1 = Lysozyme。 Madabhushi R S. Electrophoresis， 1998， 19： 224～ 230. Verzola B, Gelfi C, Righetti P G. J Chromatogr A， 2022，874： 293～ 303. 羥乙基纖維素的改性 S iOS iOS iOS iOS iOHOHOHO OO H O HN ( C H 3 ) 3 N ( C H 3 ) 3H y d r o g e n b o n dI o n i c b o n dC a t i o n i z e d h y d r o x y e t h y l c e l l u l o s eON a O HN + ( C H 3 ) 3 C l OOOO HH OO O HO H O O O HOO HO OOOO HH OOO H O O O HO
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