醫(yī)學(xué)ppt課件】信號(hào)傳導(dǎo)的病理生理學(xué)-展示頁
2025-01-17 01:13本頁面
【正文】 E 32 YKSS1 DFGLARCLASSSDSRETLVGFMTEYVATRWYRAPE 35 YFUS3 DFGLARIIDESAADNSEPTGSGMTEYVATRWYRAPE 38 domain VII VIII Loop12(TLoop) sequence of MAP kinases Southern MedicalUniversity Construction of p38 loop12 to ERK like structure p38 ...DFGLARHTDDEMTGYVATRWYRAPE... p38(E) ...DFGLARHTDDEMTEYVATRWYRAPE... p38(6+) ...DFGLARHTDDEHDHTGFMTGYVATRWYRAPE... p38(6+E) ...DFGLARHTDDEHDHTGFMTEYVATRWYRAPE... p38(VAP) ...DFGLARVADPEMTGYVATRWYRAPE... p38(DL) ...DFGLARHTDDDLTGYVATRWYRAPE... p38(VAPD6+LE) ...DFGLARVADPDHDHTGFLTEYVATRWYRAPE... ERK2 ...DFGLARVADPDHDHTGFLTEYVATRWYRAPE... Southern MedicalUniversity MAPK MKK Substrates Loop12 is a key structure to determine the selection of substrate ? ? (...TXY...) Southern MedicalUniversity 4. p38 通過磷酸化 MEF2C，調(diào)節(jié) cJun的基因表達(dá) Southern MedicalUniversity Identification of MEF2C as a substrate for p38 Southern MedicalUniversity T293,T300 and S387 of MEF2C is p38 phosphorylation sites Southern MedicalUniversity Induction of cJun through MEF2C phosphorylation by p38 Southern MedicalUniversity MKK4 MKK3 MKK6 Cytokine Genes Expression LPS p38 p38 JNK JNK MEF2C cJun MEF2C cJun MEF2 site cJun Gene Expression TNF,IL1,etc. Inflamatory Reaction AP1 p p p p p p p p p Southern MedicalUniversity 5. p38在 LPS誘導(dǎo) TNF基因表達(dá)中起著重要的調(diào)控作用 Southern MedicalUniversity 0 2 4 6 8 1 0 1 2 1 4 0 5 10 20 30 60 90 120 Time (min) Relative kinase activity Dynamic processes of p38 activation by LPS in RAW cells Southern MedicalUniversity 0 5 1 0 1 5 Relative luciferase activity Control LPS EGF LPS+FHPI p38 is involved in the enhancement of TNF? promoter transactivity Southern MedicalUniversity LPS、 UV刺激心肌細(xì)胞共聚焦顯微鏡大體掃描 LPS Control UV Southern MedicalUniversity p38 p38? p38? p38? Southern MedicalUniversity p38 p38? p38? p38? Southern MedicalUniversity Red fluorescence protein（ RFP）expression vector Southern MedicalUniversity pDsRed1N1空載體在 HeLa細(xì)胞中的表達(dá) (?800) p38?RFP融合載體在 HeLa細(xì)胞中的表達(dá) (?800) Southern MedicalUniversity
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