chapter9核糖體ribosom-文庫吧資料
2025-05-05 08:34本頁面
【正文】 Dice into Small Pieces Assemble into RISC Complex Small Inhibiting RNA (siRNAs) Perfect Basepairing to mRNA m7Gppp AAAAAA mRNA Cleavage and Destruction 21 dsRNA Dicer DNA CH3 CH3 Methylation of DNA Sequences Repression of Transcription ?其它 RNAi現(xiàn)象 ： PTGS 早在 1990年， Jensen等將帶有紫色色素的基因通過轉(zhuǎn)基因?qū)氚珷颗；?( Petunia)中 ,以期牽牛花的顏色更加艷麗。因此 RNAi技術(shù)又被形象的稱為基因敲低（ knock－ down）。 三、 RNA在生命起源中的 地位及其演化過程 生命是自我復(fù)制的體系 DNA代替了 RNA的遺傳信息功能 蛋白質(zhì)取代了絕大部分 RNA酶的功能 RNA interference RNAi scoops medical Nobel Two US geicists who discovered one of the fundamental mechanisms by which gene expression is controlled have received a Nobel prize for their achievement. Andrew Fire and Craig Mello, who revealed the process of RNA interference (RNAi) in 1998, will share the US$ award. Nature News October 2, 2022 Works of Andrew Fire and Craig Mello Background of RNAi research It’s an branch of Reverse Geics ? Have a gene in hand (genome sequence, for example), and want to know what it does. ? Potentially applicable to all anisms: no breeding necessary. protein: eliminate its functionality with an antibody cell cell DNA: homologous rebination (yeast, mice) chromosome chromosome gene marker chromosome chromosome marker RNA? cap AAAA... mRNA can no longer be translated into a protein prediction: sense RNA shouldn’t do anything... cap AAAA... mRNA: in vitro transcribe an antisense RNA Kill the messenger! Make antisense RNA Mutant Phenotype polyA Explanation: Inhibit Translation Make sense RNA Mutant Phenotype polyA Explanation: Inhibit Translation? HOW? Make antisense R
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