【正文】
為防止強(qiáng)光、高溫灼傷試管苗,在溫室頂上加蓋一層黑色遮陽(yáng)網(wǎng)。 馬鈴薯生根培養(yǎng): Stage 3 Stage 4 へ 4 weeks 発根する Rooting medium Full strength MS medium IBA mg L1 vermiculite 100ml/vessel 伸長(zhǎng)したシュート At this stage, rooted plantlets are transferred to a cell tray and grown under 60% shaded and high RH conditions for 7 to 10 days to acclimatize Acclimatization are usually attained in 2 to 3 weeks Acclimatization Acclimatization 4 stage ? From in vitro environment to ex vitro environment ? From photomixotrophy to photoautotrophy ? Carbon source: sugar in the media ? Aseptic environment ? Low light intensity ? High relative humidity ? Carbon source: CO2 in the air ? Nonaseptic environment ? high light intensity ? Low relative humidity In vitro environment Ex vivo environment Acclimatization 煉苗室溫度: 白天 23~27℃ ,夜間不低于 14℃ 。 development ? Medium ? ? Culture environment conditions? Discussions MS++D泛酸鈣 10mg +3%蔗糖; MS (surgefree medium)。 2025℃ , 30004000LX, 1416hr光照; 每 3周繼代一次,單芽莖段約 1