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煉苗具體方法 :移植前 7d左右,將長有 3~5片葉、高 2~3cm的試管苗,在不開瓶口的狀態(tài)下,從培養(yǎng)室移至溫室排好。 馬鈴薯繼代培養(yǎng)基: After culturing for 34 weeks, elongated shoots are transferred to multiplication medium Stage 2 4 weeks 分割移植 3 6 Principle of multiplication Nodal proliferation, Axillary bud formation One nodal cutting one plant,high geics,simple operation, higher survival exvitro,but lower multiplication rate at beginning. 6 3 6 shoots 6 shoots Nodal cuttings CUT CUT Axillary bud formation Restrain topbud growth, enhance formations of axilary buds High geics, simple operation Axillary branching Next explant CUT Principle of multiplication Young shoots or bud grown on a appropriate culture medium bee cluster shoots. Higher multiplication rate with higher mutation when high phytohormone used. Shoot proliferation Elongation and rooting 3 stage Shoot elongation Rooting Elongation medium Rooting medium Stage 3 A. Shoot elongation B. Root induction amp。 ? 。 馬鈴薯病毒的種類 Explant ① Geically superior ② Clea