20xx年醫(yī)學(xué)專題—-免疫原和免疫球蛋白-資料下載頁(yè)
2025-10-26 07:16本頁(yè)面
【正文】 method for obtaining a monoclonal antibody is illustrated here.,多克隆(k232。 l243。nɡ)抗體,第四十四頁(yè)，共五十一頁(yè)。,單克隆抗體制備(zh236。b232。i)流程,單克隆抗體(k224。ngtǐ),第四十五頁(yè)，共五十一頁(yè)。,To make antibodies with fully human protein sequences is to use transgenic strains of mice in which mouse antibody gene expression is suppressed and effectively replaced with human antibody gene expression. In these transgenic mice, the mouse genes for creating antibodies have been inactivated and replaced by human antibody genes. the HuMAbMouse transgenic strains contain key gene sequences from unrearranged human antibody genes that code for both the heavy and light chains of human antibodies. Because genes determine what proteins are made, the transgenic mice make human antibody proteins.,基因工程(jīyīn gōngch233。ng)抗體,第四十六頁(yè)，共五十一頁(yè)。,Chimeric and hybrid monoclonal antibodies engineered by recombinant DNA technology. (a) Chimeric mouse human monoclonal antibody containing the VH and VL domains of a mouse monoclonal antibody (blue) and CL and CH domains of a human monoclonal antibody (gray). (b) A chimeric monoclonal antibody containing only the CDRs of a mouse monoclonal antibody (blue bands) grafted within the framework regions of a human monoclonal antibody is called a “humanized〞 monoclonal antibody. (c) A chimeric monoclonal antibody in which the terminal Fc domain is replaced by toxin chains (white). (d) A heteroconjugate in which one half of the mouse antibody molecule is specific for a tumor antigen and the other half is specific for the CD3/Tcell receptor complex.,嵌合(qi224。n h233。)抗體和改型抗體,第四十七頁(yè)，共五十一頁(yè)。,人源化抗體的種類 鼠源抗體對(duì)于(du236。y)人具有很強(qiáng)的免疫原性，限制了臨床使用；將鼠源抗體可變區(qū)以外局部換成人源，為嵌合抗體； 僅保存可變區(qū)CDR區(qū)為鼠源氨基酸的抗體為人源化抗體；為改善CDR區(qū)域的空間構(gòu)象，將局部人源FR區(qū)域氨 基酸置換為鼠源，可增強(qiáng)抗體親和力。,第四十八頁(yè)，共五十一頁(yè)。,抗體活性片段衍生的多種基因工程抗體 根據(jù)(gēnj249。)Fv抗體 (VH,VL) (A)、Fab抗體(B) 和單鏈抗體(ScFv) (C) 分別構(gòu)建的多種基因工程抗體。,抗體片段及其衍生(yǎn shēnɡ)形式,第四十九頁(yè)，共五十一頁(yè)。,復(fù)習(xí)(f249。x237。)思考題,免疫原的分類和影響因素 免疫球蛋白根本結(jié)構(gòu)和生物學(xué)功能 抗體制備(zh236。b232。i)、種類和應(yīng)用價(jià)值,第五十頁(yè)，共五十一頁(yè)。,內(nèi)容(n232。ir243。ng)總結(jié),免疫(miǎny236。)原和免疫(miǎny236。)球蛋白。固有分子模式涵蓋PAMP、DAMP及其它已發(fā)現(xiàn)或有待確認(rèn)的各種模式成份。G+和G細(xì)菌胞壁中的多種PAMP。T細(xì)胞表位肽的同時(shí)，必須同時(shí)識(shí)別MHC分子。?TLR的天然配體和沖動(dòng)劑?參見表44。為改善CDR區(qū)域的空間構(gòu)象，將局部人源FR區(qū)域氨?？贵w制備、種類和應(yīng)用價(jià)值,第五十一頁(yè)，共五十一頁(yè)。
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