光學(xué)顯微與電子成像1-資料下載頁(yè)
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【正文】 3 44 What is Contrast（對(duì)比度） ? Differences in light intensity, phase, polarization, color create image contrast, which allows individual features and details of the specimen to bee visible. Contrast is defined as the difference in light intensity between the object and the adjacent background relative to the overall background intensity. Percent Contrast = [(I(s) I(b))] / I(b) x 100% 45 Contrast Mechanisms（都有哪些對(duì)比度） ? Bright field (low contrast) 明場(chǎng) ? Phase contrast 相位襯度 ? Differential interference contrast (DIC) 微分干涉 ? Dark field contrast 暗場(chǎng) ? Polarization microscopy 偏振顯微 ? Fluorescence microscopy Chemical Selectivity ? Vibration microscopy 振動(dòng)顯微 46 Summarization 1. Optical Microscope is a powerful tool in many fields including life science, clinics, material and chemistry. 2. K246。hler Illumination: standard for designing an optical microscope 3. Components of a microscope: – Objective lens (Aberration correction, NA, working distance) – Detectors (CCD, PMT) 4. Contrast mechanisms – Phase contrast – DIC contrast – Dark field contrast – Fluorescence 5. Reference: 47 Quiz 1. Who invented the first microscope? 2. What are the goals of microscopy? 3. What are the main ponents of an optical microscope? 4. What is the overall magnification of the microscope? 5. Do you have any experience with microscopy? Please describe. 6. How are the microscopes classified in terms of their structures? 7. What is the contrast of optical microscope? Please give a definition and its expression using an equation. 8. What are the mon contrast mechanisms for optical microscopy? 謝謝觀看 /歡迎下載 BY FAITH I MEAN A VISION OF GOOD ONE CHERISHES AND THE ENTHUSIASM THAT PUSHES ONE TO SEEK ITS FULFILLMENT REGARDLESS OF OBSTACLES. BY FAITH I BY FAITH
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