光學(xué)顯微與電子成像1(存儲(chǔ)版)
【正文】 ocular)目鏡 ? An eyepiece, or ocular lens: is a type of lens that is attached to a variety of optical devices such as telescopes（望遠(yuǎn)鏡 ) and microscopes(顯微鏡） . named because it is usually the lens that is closest to the eye A collection of different types of eyepieces A 25 mm Kellner eyepiece 12 What is eyepiece (or ocular)? An eyepiece is usually described by barrel diameter and focal length. Common barrel diameters are , (standard) and 2. Commonly available eyepiece focal lengths range from 2mm to 40mm. 1Real image 2 Field diaphragm 3 Eye relief 4 Exit pupil The eyepiece is placed at the focal point of the objective 13 What is objective? Objective: the optical element that gathers(收集 ) light from the object being observed and focuses the light rays to produce a real image(實(shí)像） . named because it is usually the lens that is closest to the object Leica oil immersion microscope objective 14 15 How image bees magnified in microscope? 16 How image bees magnified in microscope? 17 How image bees magnified in microscope? 18 How image bees magnified in microscope? 19 How image bees magnified in microscope? 20 Basic Configuration: Inverted Microscope（ 倒置顯微鏡） inverted microscope for fluorescence microscopy（倒置熒光顯微鏡） . Inverted microscope: light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. inverted microscope for tissue culture（組織培養(yǎng)用倒置顯微鏡） . 21 Basic Configuration: Upright Microscope（ 正置顯微鏡） Upright microscope: light source and condenser on the bottom, below the stage pointing up, while the objectives and turret are above the stage pointing down. 22 Principle of Koeher Illumination: ? A given point in the specimen is
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