光學(xué)顯微與電子成像1(已修改)
2025-01-08 13:35 本頁面
【正文】 Fundamentals of Light Microscopy Junle Qu Email: 深圳大學(xué)光電工程學(xué)院 Chapter 1 2 Microscope顯微鏡 The oldest published image known to have been made with a microscope (1630) ? It is often said that Dutch spectaclemakers Hans Janssen and his son Zacharias Janssen to invent the first pound microscope in 1600s Microscope: instrument to see objects too small for the naked eye. ? It is difficult to say who invented the pound microscope. History of invention 3 Three Goals of Microscopy ?M: produce a magnified image of the specimen ?R: separate the details in the image ?C: render the details visible to the human eye or camera. MRC: Magnification, Resolution, Contrast 4 History of Light Microscopy A simple pound microscope invented by British microscopist Robert Hooke sometime in the 1660s. 5 6 7 History of Light Microscopy 8 Invented by Robert Hooke in1600s Microscope 20th century microscopes 21th century microscopes 9 Microscope Microscopy Microscopic 1. Microscope (顯微鏡 ) instrument to see objects too small for the naked eye. 2. Microscopy (顯微成像方法 ) the science of investigating small objects using such an instrument. 3. Microscopic (用顯微鏡可見的 ) invisible to the eye unless aided by a microscope. 10 Microscope Elements ? Ocular lens (eyepiece) (1)目鏡 ? Objective (3) –物鏡 ? Diaphragm and condenser lens (8) 光欄和聚光鏡 ? Light source, a light or a mirror (7) Basic optical transmission microscope elements ? Objective turret (2) （目鏡架） ? Stage (to hold the sample) (6 ) 、 ( 9) 樣品臺(tái) ? Focus wheel（聚焦輪） to move the stage (4 coarse adjustment, 5 fine adjustment) Basic ponents of the light path：光路部分構(gòu)成 “Structural” ponents:結(jié)構(gòu)部分 11 What is an eyepiece (or
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