三種測(cè)序dna模板制備方法的比較-資料下載頁(yè)
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【正文】 7):49654972.Li P, Cao RB, Zheng QS, et al. Enhancement of humoral and cellular immunity in mice against Japanese encephalitis virususing a DNA primeprotein boost vaccine strategy. Vet J. 2010。183(2):210216.Yang Y, Hebron HR, Hang J. A method for preparing DNAsequencing templates using a DNAbinding microplate. J BiomolTech. 2009。20(3):165171.Hjelm LN, Chin EL, Hegde MR, et al. A simple method to confirmand size deletion, duplication, and insertion mutations detected by sequence analysis. J Mol Diagn. 2010。12(5):607610.Whetten RW, Sof237。a VA, Frampton J. Polymerase chain reactionpreparation of template for massively parallel pyrosequencing. J Biomol Tech. 2009。20(2):128134.Juan C, Armando A. Determination of the in vivo structural DNAloop organization in the genomic region of the rat albumin locus by means of a topological approach. DNA Res. 2010。17(1):2335. Qi X, Wu Q, Zhang Y, et al. A novel model for DNA sequencesimilarity analysis based on graph theory. Evol Bioinform Online.2011。7:149158.Casci T. DNA sequencing: Exome sequencing technologiespared. Nat Rev Genet. 2011。12(11):741.Yang X, Jiao R, Yang L, et al. Yichuan. 2010。33(8):829846. 楊旭,焦睿,楊琳, 略[J].遺傳, 2010,33(8):829846.Pareek CS, Smoczynski R, Tretyn A. Sequencing technologies and genome sequencing. J Appl Genet. 2011。52(4):413435. Mardis ER. Nextgeneration DNA sequencing methods. Annu Rev Genomics Hum Genet. 2008。9:387402.Xiao L, Zhang J, Sirois P, et al. A new strategy for next generation sequencing: merging the Sanger39。s method and the sequencing bysynthesis through replacing extension. J Biomed Nanotechnol.2011。7(4):568571.[21][2][22][3][23][4][24][5][25][6][26][7][8][9][10][11][12][13][14][15][16]2822萬(wàn)方數(shù)據(jù). Box 1200, Shenyang110004 來(lái)自本文課題的更多信息作者貢獻(xiàn)：實(shí)驗(yàn)設(shè)計(jì)為通訊作者，實(shí)驗(yàn)實(shí)施為第一、二 三作者，評(píng)估為所有作者，均經(jīng)過系統(tǒng)培訓(xùn)。第一作者對(duì)文 章負(fù)責(zé)。利益沖突：課題未涉及任何廠家及相關(guān)雇主或其他經(jīng)濟(jì) 組織直接或間接的經(jīng)濟(jì)或利益的贊助。本文創(chuàng)新性：課題設(shè)計(jì)的創(chuàng)新之處在于技術(shù)創(chuàng)新，即為 基因組 DNA 或甲基化 DNA 測(cè)序?qū)ふ乙环N經(jīng)濟(jì)，簡(jiǎn)便的方 法。實(shí)驗(yàn)所用到的 3 種方法均適用于基因組 DNA 的測(cè)序， 而 96 管集合板法更適用于甲基化 DNA 的測(cè)序。
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