三種測序dna模板制備方法的比較(存儲版)
【正文】 序， 而 96 管集合板法更適用于甲基化 DNA 的測序。33(8):829846. 楊旭,焦睿,楊琳, 略[J].遺傳, 2010,33(8):829846.Pareek CS, Smoczynski R, Tretyn A. Sequencing technologies and genome sequencing. J Appl Genet. 2011。183(2):210216.Yang Y, Hebron HR, Hang J. A method for preparing DNAsequencing templates using a DNAbinding microplate. J BiomolTech. 2009。1:4446. 宋艷斌,馬文麗,[J].生物技 術(shù)通報,2005,1:4446.序失敗。22(2):5359.Pardo CE, Carr IM, Hoffman CJ, et al. MethylViewer: putational analysis and editing for bisulfite sequencing and methyltransferase accessibility protocol for individual templates(MAPit) projects. Nucleic Acids Res. 2011。而3種方法在基因組DNA測序時， 均能獲得較好的效果。然而，在DNA測序中也存在一些問題，比如DNA模板質(zhì)量、循環(huán)測序 反應(yīng)條件和變性凝膠電泳條件等，這些都可能 降低測序的效能，直接影響著結(jié)果的準(zhǔn)確性及 重復(fù)性[1819]。法三之間比較差異有顯著性意義(χ2=，P= )。甲基化 DNA 準(zhǔn)備：用甲基化試劑盒對提純的 DNA 進行甲基化處理。實驗擬通過測序 結(jié)果對3種測序DNA模板制備方法進行比較，旨 在獲得一種簡便、經(jīng)濟、高效的測序方法。Corresponding author: Liu Shiguo, Doctor, Professor, Laboratory of Molecular Biology, Xinxiang Medical University, Xinxiang453003, Henan Province, China lsg關(guān)鍵詞：基因組 DNA；甲基化 DNA；模板；DNA 測序doi:秦川，張俊河，Robert ，劉世國. 三種測序 DNA 模板制備方法的比較[J].中國組織工程研究，2012，16(15):28202822.[ ]Received: 20110925Accepted: 20111020時間及地點：于201008/201104在美國猶他州大學(xué)分子生物學(xué)實驗室完成。中國組織工程研究 第 16 卷 第 15 期 2012–04–08 出版Chinese Journal of Tissue Engineering Research April 8, 2012 , 三種測序DNA模板制備方法的比較★○秦 川1，張俊河2，Robert A. Mcknight3○，劉世國1Comparison of three methods for preparing DNA sequencing templatesQin Chuan1, Zhang Junhe2, Robert A. Mcknight3○, Liu Shiguo1AbstractBACKGROUND: The quality of DNA templates plays a crucial role in DNA sequencing.OBJECTIVE: To provide an economical, simple method for genomic DNA sequencing and methylated DNA sequencing. METHODS: Plasmid extraction was performed using NucleoSpin multi96 plus plasmid kit and edgebio kit SeqPrep? 96 HP plasmid prep system, respectively. Primer pairs containing target fragments were designed for the plasmids. PCR products were purified after amplification. DNA sequencing templates were prepared by the three methods above.RESULT
規(guī)章制度相關(guān)推薦
鄂ICP備17016276號-1