microrna論文：低溫環(huán)境下microrna對小鼠腦外傷后海馬區(qū)神經(jīng)干細胞-資料下載頁
2025-06-22 16:19本頁面
【正文】 。, P)。 (3) the number of Notchl+ cells in the ipsilateral hippocampal DG of LTBI 3 days group was less pared to NTBI 3 days group (P), suggesting TBI can induce Notch1 protein upregulation, whereas low temperature can inhibit its Endogenous NSCs proliferation:Immunofluorescence show that, (1) the number of Nestin+ cells in the ipsilateral hippocampal DG of NTBI 3 days group was significantly enhanced pared to NTBI sham group (177。 vs 177。, P)。 (2) the number of Nestin+ cells in the ipsilateral hippocampal DG of LTBI 3 days group was also significantly enhanced pared to LTBI sham group (177。 vs 177。, P)。 (3) the number of Nestin+ cells in the ipsilateral hippocampal DG of LTBI 3 days group was less pared to NTBI 3 days group (P), suggesting TBI can induce NSCs proliferation in DG, whereas low temperature can inhibit its :1. Lots of miRNAs (such as miR200b and miR34a) expression were changed in the hippocampus after TBI, whereas low temperature can affect their expression post . TBI can significantly decrease miR34a expression, increase its target Notch1 signaling expression and induce NSCs proliferation in the hippocampus, suggesting miR34a/Notch1 pathway might be involved in traumainduced NSCs . Low temperature exposure might affect NSCs proliferation by inhibiting the role of miR34a/Notch1 pathway in the hippocampus after TBI, suggesting low temperature exposure may affect TBI prognosis.【關(guān)鍵詞】microRNA 神經(jīng)干細胞 Notch1 腦外傷 低溫 基因芯片【英文關(guān)鍵詞】microRNA neural stem cells Notch1 traumatic brain injury low temperature microarray【目錄】低溫環(huán)境下microRNA對小鼠腦外傷后海馬區(qū)神經(jīng)干細胞增殖分化的調(diào)控作用摘要37ABSTRACT711第1章 引言1417第2章 材料與方法1731 實驗材料1719 實驗方法1931第3章 結(jié)果3138 閉合性腦外傷小鼠模型的建立31 腦外傷后海馬區(qū)MIRNA表達譜的變化3133 總RNA質(zhì)量鑒定31 腦外傷后海馬區(qū)miRNA表達譜的變化3133 realtime PCR驗證芯片結(jié)果33 腦外傷后海馬區(qū)MIR34A、NOTCH1信號分子的表達變化和內(nèi)源性NSCs增殖情況3338 miR34a的表達變化34 Notch1信號分子的表達變化3436 內(nèi)源性NSCs的增殖情況3638第4章 討論3849 腦外傷動物的低溫暴露處理3839 NSCs與腦外傷3940 MIRNA與NSCs4043 腦損傷后NSCs增殖分化相關(guān)MIRNA的表達變化4345 MIR34A/NOTCH1信號通路對腦外傷后海馬區(qū)NSCs增殖的調(diào)控作用4549第5章 結(jié)論4950致謝5051參考文獻5157附圖5760攻讀學(xué)位期間的研究成果6061綜述6172參考文獻697213 / 13
環(huán)評公示相關(guān)推薦
鄂ICP備17016276號-1