應(yīng)用ssap技術(shù)分析漸滲系后代多樣性研究畢業(yè)論文-資料下載頁
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【正文】 , Han F, Dong Y, Liu B. Mobilization of the active MITE transposons mPing and Pong in rice by introgression from wild rice (Zizania latifolia Griseb.) [J]. Molecular Biology and Evolution, 2005, 22(4): 976990.[9] Kashkush K, Feldman M, Levy AA. Transcriptional activation of retrotransposons alters the expression of adjacent genes in wheat[J].Nature Genetics,2003,33(1):102106.[10] Kumar A, Pearce SR, McLean K, et al. The Ty1copia group of retrotransposons in plants: genomic organization, evolution and use as molecular markers [J]. Genetica, 1997, 100(13): 205217. [11] Kumar A. The adventures of the Ty1copia group of retrotransposons in plants [J].Trends in Genetics,1996,12(2):4143. [12] Kumar A, Bennetzen JL. Plant retrotransposons [J].Annual Review of Genetics,1999,33: 479532. [13] Grandbastein MA. Activation of plant retrotransposons under stress conditions [J].Trends in Plant Science,1998,3(5):181187.[14] Ostertag EM, Kazazian HH. Biology of mammalian L1 retrotransposons [J].Annual Review of Genetics,2001,35:501538.[15] Pearce SR, Harrison G, Li D, et al. The Ty1copia group retrotransposons in Vicia species: copy number, sequence heterogeneity and chromosomal localisation [J].Molecular and General Genetics,1996b,250(3):305315. [16] Mhiri C, De Wit PJ, Grandbastien MA. Activation of the promoter of the Tnt1 retrotransposon in tomato after inoculation with the fungal pathogen Cladosporium fulvum [J].Molecular PlantMcrobe Interactions,1999,12(7):592603.[17] Grandbastien MA, Lucas H, Morel JB, et al. The expression of the tobacco Tnt1 retrotransposon is linked to plant defense responses [J].Genetica, 1997,100(13): 241252. [18] Boeke JD,Corces VG. Transcription and reverse transcription of retrotransposons [J]. Annual Review in Microbiology, 1989, 43: 403434. [19] Waugh R, McLean K, Flavell S, et distribution of Bare 1like retrotransposable elements in the barley genome revealed by sequencespecific amplification polymorphisms (SSAP) [J]. Molecular and General Genetics, 1997, 253(6): 687694. [20] Berenyi M, Gichuki ST, Schmidt J, et al. Ty1copia retrotransposonbased SSAP (sequencespecific amplified polymorphism) for genetic analysis of sweetpotato [J]. Theoretical and Applied Genetics, 2002, 105(67): 862869. [21] Tam SM, Mhiri C, Vogelaar A, et al. Comparative analyses of genetic diversities within tomato and pepper collections detected by retrotransposonbased SSAP, AFLP and SSR [J]. Theoretical and Applied Genetics, 2005, 110(5): 819831. [22] Lou QF, Chen JF. Ty1copia retrotransposonbased SSAP marker development and its potential in the genetic study of cucurbits [J]. Genome, 2007, 50(9): 802810. [23] Kalendar R, Grob T, Regina M, et al. IRAP and REMAP: two new retrotransposonbased DNA fingerprinting techniques [J]. Theoretical and Applied Genetics, 1999, 98(5): 704711. [24] 劉亞今, 李作洲, 姜正旺, [J].植物生態(tài)學(xué)報(bào), 2008, 32(3), 704718.[25] 鄧曉娟, 東鄉(xiāng)野生稻基因漸滲系的DNA 序列及甲基化變化研究,2012.[26] Murray HG, Thompson WF. Rapid isolation of higher weight DNA [J]. Nucleic Acids Research, 1980, 8(19): 43214326. [27] 簡(jiǎn)水溶, 萬勇, 羅向東, 方軍, 儲(chǔ)成才, 謝建坤. 東鄉(xiāng)野生稻苗期耐冷性的遺傳分析 [J]. 植物學(xué)報(bào), 2011, 46(001): 2127.附錄A 主要實(shí)驗(yàn)儀器離心管、研缽、水浴鍋、離心機(jī)、冰柜、冰箱、PCR儀、電泳槽、微波爐、凝膠成像分析系統(tǒng)、脫色搖床、電子天平、微量移液器、手提式壓力蒸汽滅菌器蒸餾水、液氮、CTAB、氯仿、異戊醇、10%乙醇、70%乙醇、75%乙醇、無水乙醇、TE溶液、RNaseA、ddH2O、醋酸鈉、EcoR I、Mse I 、10T4 ligase Buffer、EcoR I 接頭、Mse I 接頭、T4 DNA ligase、MgCldNTP、 Taq 聚合酶、尿素、40%PA膠貯液、TBE、四甲基乙二胺、10%過硫酸銨、預(yù)擴(kuò)增和選擇性擴(kuò)增引物、甲酰胺、%冰醋酸、硝酸銀染液符號(hào)表AFLP 擴(kuò)增片段長(zhǎng)度多態(tài)性(Amplified Fragments Length Polymorphism)BIL 回交重組自交系(Backcross Inbred Line)DWR 東鄉(xiāng)野生稻(Dongxiang Wild Rice)ILs漸滲系(Introgression lines)PBS引物結(jié)合位點(diǎn)（primer binding site）PCA主成分分析(Principal coordinate analysis)PCR 聚合酶鏈?zhǔn)椒磻?yīng)(Polymerase Chain Reaction)SSAP序列擴(kuò)增多態(tài)性（Sequencespecific amplification polymorphism）SSR簡(jiǎn)單重復(fù)序列(Simple Sequence Repeat）XB 栽培稻協(xié)青早B(Xieqingzao B)23致謝本研究及學(xué)位論文是在羅向東老師的親切關(guān)懷和悉心指導(dǎo)下完成的。從老師身上，我學(xué)到的不僅僅是豐富的專業(yè)知識(shí)，更學(xué)到了嚴(yán)謹(jǐn)?shù)目茖W(xué)態(tài)度。在此論文完成之際，我真誠地對(duì)羅老師致以深深的謝意！特別感謝陳雅玲學(xué)姐給我細(xì)心的指導(dǎo)和不懈的支持。再次致上我無限的謝意和感激！
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