生物質(zhì)譜分析技術(shù)ppt課件-資料下載頁
2025-05-04 06:40本頁面
【正文】 meters, acquires signal vs. time, and processes the data 5. Ions strike the detector at different times, depending on their mass/charge ratio 質(zhì)譜工作流程 4800圖譜 7 9 9 . 0 1 4 4 1 . 8 2 0 8 4 . 6 2 7 2 7 . 4 3 3 7 0 . 2 4 0 1 3 . 0M a s s ( m / z )1 . 3 E + 40102030405060708090100% Intensity4 7 0 0 R e f l e c t o r S p e c 1 M C [ B P = 1 5 7 2 . 9 , 1 3 1 9 9 ]1571.88721889.95231946.0353869.35881797.91112582.25732761.37182197.19651876.95673619.75291811.8799855.05811156.60931960.04281707.79612102.1436945.47441381.69621320.58581593.87122383.92902603.27321493.76882315.15971232.63642535.25612678.26271049.50152021.96283312.36352831.21362757.35742924.40823841.67583680.78493776.71093188.91823050.3032PMF圖譜 9 . 0 4 6 9 . 8 9 3 0 . 6 1 3 9 1 . 4 1 8 5 2 . 2 2 3 1 3 . 0M a s s ( m / z )6 . 0 E + 40102030405060708090100% Intensity4 7 0 0 M S / M S P r e c u r s o r 2 1 9 0 . 5 1 S p e c 1 M C [ B P = 2 1 8 7 . 7 , 6 0 1 4 8 ]2 1 8 7 . 6 6 3 82 0 6 1 . 7 0 5 81 2 7 1 . 5 1 8 86 7 7 . 2 4 2 42 0 9 8 . 8 6 1 31 5 1 3 . 5 5 4 71 1 8 5 . 3 7 7 85 9 0 . 2 0 8 62 0 4 6 . 6 4 4 34 7 7 . 1 2 3 9 1 6 0 0 . 5 9 2 31 0 0 5 . 4 0 2 81 2 5 4 . 5 0 4 38 4 5 . 3 4 0 4 2 0 3 3 . 7 4 5 01 1 2 5 . 4 3 4 96 6 0 . 1 9 6 21 3 4 5 . 4 0 0 4 2 0 1 9 . 8 9 2 23 0 0 . 0 6 6 51 5 1 7 . 3 9 9 99 1 4 . 3 7 5 92 0 5 0 . 2 6 7 14 6 0 . 0 8 0 16 9 5 . 2 4 9 51 7 1 3 . 6 8 4 11 2 8 9 . 5 4 3 2 1 4 9 5 . 5 4 1 0 2 1 0 5 . 2 9 4 71 1 0 . 0 8 0 0 8 6 3 . 3 5 6 54 6 7 . 0 9 7 5 1 9 0 1 . 7 4 6 06 3 2 . 2 0 3 41 1 1 8 . 2 9 1 6 1 4 7 7 . 2 0 8 41 2 9 5 . 3 8 7 62 6 7 . 0 8 1 87 0 . 0 8 8 8 1 6 7 7 . 7 6 9 39 5 5 . 3 0 4 54 1 8 . 0 5 7 4 7 9 6 . 2 7 4 96 2 7 . 0 6 7 6PST圖譜 A: 質(zhì)譜圖中最強(qiáng)子的相對強(qiáng)度 (Y axis) B: 離子的質(zhì)量與電荷比 (m/z, X axis) C:離子峰稱為基峰 (base peak) D: 相對于特定的檢測器時稱為峰的絕對強(qiáng)度 E: 所有質(zhì)譜峰對應(yīng)的是離子電信號強(qiáng)度和離子應(yīng)在的 m/z位置 F: 棒狀譜 (centroidal peak) G: 高斯峰 (gauss peak) F 質(zhì)譜圖意義 G 生物質(zhì)譜技術(shù)的應(yīng)用 ? ? ? ? 思考題： 4. MALDI電離方法的原理 5. 生物質(zhì)譜技術(shù)的主要應(yīng)用領(lǐng)域
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