級檢驗英語ppt課件(2)-資料下載頁
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【正文】 ailable, the assay can determine the absolute amount of antigen in an unknown sample. The sandwich ELISA requires two antibodies that bind to epitopes(表位 ) that do not overlap on the antigen. Sandwich ELISA This can be acplished with either two monoclonal antibodies that recognize discrete (不連續(xù)的 ) sites or one batch of affinitypurified polyclonal antibodies. To utilize this assay, one capture antibody is purified and bound to a solid phase typically attached to the bottom of a plate well. Antigen is then added and allowed to plex with the bound antibody. Unbound products are then removed with a wash, and a labeled second detection antibody is allowed to bind to the antigen, thus pleting the sandwich. Sandwich ELISAThe assay is then quantitated by measuring the amount of labeled second antibody bound to the matrix (矩陣 ), through the use of a colorimetric substrate. Major advantages of this technique are that the antigen does not need to be purified prior to use, and that these assays are very specific. However, one disadvantage is that not all antibodies can be used. Monoclonal antibody binations must be qualified as unattached pairs, meaning that they can recognize separate epitopes on the antigen so they do not hinder each other binding.Sandwich ELISA
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