代謝引論和生物能學(xué)概述-資料下載頁(yè)
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【正文】 e is MgATP2 ATP在能量轉(zhuǎn)運(yùn)中地位和作用 ★ ATP是細(xì)胞內(nèi)的 “ 能量通貨 ” ★ ATP是細(xì)胞內(nèi)磷酸基團(tuán)轉(zhuǎn)移的中間載體 ～ P ～ P ～ P ～ P ATP ～ P 0 2 10 8 6 4 12 14 磷酸基團(tuán)轉(zhuǎn)移能 磷酸烯醇式丙酮酸 3磷酸甘油酸磷酸 磷酸肌酸 （磷酸基團(tuán)儲(chǔ)備物） 6磷酸葡萄糖 3磷酸甘油 ATP provides energy by group transfer (donating a Pi, PPi or AMP to form covalent intermediates), not by simple hydrolysis ? ATP energizes active transport and muscle contraction. ? In the firefly, ATP is used in a set of reactions that convert chemical energy into light energy. ? In the lab, as little as a few pioles (1012 mol) of ATP can be measured using firefly luciferin and luciferase (熒光素酶 ), using spectroscopic methods. In the firefly, ATP is used in a set of reactions that convert chemical energy into light energy ? ATP is not a longterm storage form of free energy in living cells, being consumed within a minute following its formation (phosphocreatine(磷酸肌酸 ) act as a energy storage form for longer term). ? The concentration of ATP remains constant, because the rate of ATP consumption equals its rate of synthesis. ? A resting human consumes about 40 kg of ATP in 24 hours! References ? Alberty, R. A. (1994) “Biochemical thermodynamics” Biochim. Biophys. Acta, 1207:111. ? Frey, P. A. and Arabshahi, A. (1995) “Standard free energy change for hydrolysis of the ab phosphoanhydride bridge in ATP” Biochemistry, 34:1130711310. ? Westheimer, ., (1987) “Why nature chose phosphates” Science,235:11731178. ? Kornberg, A et al. (1999) “Inanic polyphosphate: a molecule of many functions” Annu. Rev. Biochem. 68:89125.
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