waters公司的uplc-tqd培訓(xùn)資料ms7校正-資料下載頁
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【正文】 other folder for later use if needed. For example, you can load ‘Uncal’ and practice changing the mass measure parameters. Then choose the ‘Recalibrate From File’ option and select one of your saved files. 169。2022 Waters Corporation 54 AutoTune Wizard – Calibration 169。2022 Waters Corporation 55 AutoTune Wizard – Calibration 169。2022 Waters Corporation 56 AutoTune Wizard – Calibration 169。2022 Waters Corporation 57 AutoTune Wizard – Calibration 169。2022 Waters Corporation 58 AutoTune Wizard – Calibration 169。2022 Waters Corporation 59 AutoTune Wizard – Calibration 169。2022 Waters Corporation 60 AutoTune Wizard – Calibration 169。2022 Waters Corporation 61 Calibrate Check 169。2022 Waters Corporation 62 325 350 375 400 425 450 475 500 525 550 575 600 625 650 675 700m/z0100%PEG_1000 1 ( 0. 559) S c a n E S + 1 . 4 1 e 85 3 3 .45 1 1 .34 8 9 .44 6 7 .34 4 5 .44 2 3 .34 0 1 .25 5 5 .3 5 7 7 .45 9 9 .46 2 1 .56 4 3 .66 6 5 .5 6 8 7 .4D ou bly C ha rg e A m m on iumA dd uc t s of P E G 10 00IHGFAB CDE850 900 950 1000 1050 1100 1150 1200m/z0100%PEG_1000 1 ( 0. 559) S c a n E S + 1 . 0 7 e 89 1 6 .58 7 2 .58 2 8 .59 6 0 .61 0 0 4 .61 0 4 8 .71 0 9 2 .61 1 3 6 .61 1 8 0 .7S ing ly C ha rg e A m m on iumA dd uc t s of P E G 10 00ABCIHGFDEPEG 1000 with Ammonium Acetate 169。2022 Waters Corporation 63 Sodium Iodide Solution for Positive Ion Electrospray ? Prepare a solution of sodium iodide at a concentration of: — 2 181。g/181。L (micrograms/microliter) (2 mg/mL) in 50:50 propran2ol (IPA)/water with no additional acid or buffer. —Add cesium iodide to a concentration of 181。g/181。L (micrograms/microliter) ( mg/mL). o Cesium iodide is to obtain a peak at m/z 133 (Cs+). ? Without this, there is a gap in the calibration file of 150 amu from m/z 23 (Na+) and the first cluster at m/z 173, which leads to poor mass calibration in this mass range. ? Do not add more CsI than suggested, as it can result in a more plex spectrum due to the formation of NaCsI clusters. ? Use reference file: 169。2022 Waters Corporation 64 Sodium Iodide Solution for Positive Ion Electrospray (Alternative) ? Prepare a solution of sodium iodide, as described previously, but instead of adding cesium iodide, use rubidium iodide at a concentration of 181。g/181。L (micrograms/microliter) ( mg/mL). —This gives a reference peak at m/z 85 (85Rb) with an intensity of approximately 10% of the base peak at m/z 173. o The advantage of Rubidium Iodide is that rubidium clusters are not formed, which can plicate the spectrum. ? Use reference file: 169。2022 Waters Corporation 65 Calibration Solutions ? PEG solution for positive ion electrospray and APCI —Prepare a solution of the following polyethylene glycols in 50:50 acetonitrile/ M ammonium acetate in water (50:50 ACN/2 mM NH4Acetate in Water). o PEG 200 at 25 ng/181。L, PEG 400 at 50 ng/181。L o PEG 600 at 75 ng/181。L o PEG 1000 at 250 ng/181。L ? This produces a series of PEG+ ammonium ions allowing calibration up to approximately 1200 amu. ? To calibrate further up the mass scale, add PEG 1500 or preferably use a sodium iodide solution. ? Use reference file: 169。2022 Waters Corporation 66 Calibration Solutions ? Horse heart myoglobin for positive ion electrospray —For use when measuring large multiply charged proteins, prepare a 5 181。M solution of horse heart myoglobin in % formic acid in 50/50 water/acetonitrile. ? Use reference file:
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