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0。 培養(yǎng)條件:37℃,LB 539。 測序引物:T7:TAATACGACTCACTATAGGG 339。 測序引物:T7Ter:TGCTAGTTATTGCTCAGCGG 備注:pET30a大腸桿菌表達(dá)載體, pET30a質(zhì)粒,菌株載體簡介: The maps for pET30b(+) and pET30c(+) are the same as pET30a(+) (shown) with the following exceptions: pET30b(+) is a 5