mir396在豆科植物苜蓿根中對其菌根化和分生組織活性的(編輯修改稿)
2025-03-28 23:52 本頁面
【文章內(nèi)容簡介】 argets, we generated roots expressing a target mimicry construct that was successfully designed to hinder miR396 activity in A. thaliana. Mimicry (MIM) transcripts specifically trap members of an miRNA family, and thus prevent their activity on endogenous targets 2023/3/29 16 2023/3/29 17 Levels of transcripts of miR396 targets were measured using quantitative RTPCR in roots inactivated for miR396 activity (MIM396) 除了 GRF6，其他基因表達(dá)水平都上升，這里還不能排除有其他因子對 GRF6進(jìn)行調(diào)節(jié)的可能，本文中沒有深入研究 ? miR396 negatively affects root growth, RAM size and cell proliferation in the root apex ? To investigate the function of miR396 in roots, we measured the length and dry weight of roots that either overexpressed the mtrmiR396b precursor (miR396OE) or were inactivated for miR396 activity (MIM396). 2023/3/29 18 2023/3/29 19 miR396OE 中主根長度和干重明顯減少，而 MIM396中根長稍微增加，而干重卻大量增加。說明miR396過表達(dá)能抑制根的生長 2023/3/29 20 Root meristem size is reduced in miR396OE posite plants 2023/3/29 21 兩者之間沒有大的變化，說明miR396對細(xì)胞從細(xì)胞周期進(jìn)入分裂周期的開關(guān)沒有大的影響 2023/3/29 22 The expression of mitotic cell cycle (CYCB1。1, CYCB1。3, CYCB2。1, CYCD3。1, histone H4, CDC16) or endocycle (CCS52B) markers was measured using quantitative RTPCR in miR396OE and control roots The percentage of cells replicating (. EdUpositive nuclei) obtained from 20 root tips per construct in four independent experiments ? Together, these data strongly suggest that miR396dependent modulation of root growth may essentially be due to a restriction of cell division activity. 2023/3/29 23 ? Silencing of MtGRF genes mimics the miR396OE root growth phenotype ? To study the role of the miR396 targets on root growth, we inactivated them using an RNAi strategy. ? First, we generated posite plants expressing three RNAi constructs targeting a conserved region of various GRF genes ( MtGRF2, MtGRF4 and MtGRF6). ? In addition, we prepared an RNAi vector targeting both MtbHLH79 genes and confirmed t
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