基因工程探索性綜合性實驗一龍須菜中半乳糖-1-磷酸尿苷酰轉(zhuǎn)(編輯修改稿)
2025-01-24 02:10 本頁面
【文章內(nèi)容簡介】 tenuistipitata var. liui China Gracilaria tenuistipitata var. liui Philippines Gracilaria tenuistipitata var. liui Thailand Gracilaria tenuistipitata var. liui Vietnam Gracilaria verrucosa Argentina Gracilaria verrucosa Egypt Gracilaria verrucosa France Gracilaria heteroclada Philippines Gracilaria heteroclada Vietnam Gracilaria howei Peru Gracilaria lemaneiformis Japan Gracilaria lemaneiformis Mexico Gracilaria lemaneiformis Peru Gracilaria longa Italy Gracilaria pacifica Canada Gracilaria parvispora United States of America Gracilaria salicornia Thailand Gracilaria salicornia Vietnam Gracilaria spp. Burma Gracilaria asisatica China Gracilaria asisatica Vietnam Gracilaria bursapastoris Japan Gracilaria caudata Brazil Gracilaria changii Thailand Gracilaria chilensis Chile Gracilaria chilensis New Zealand Gracilaria cornea Brazil Gracilaria cornea Caribbean Gracilaria coronopifera United States of America Gracilaria coronopifera Vietnam Gracilaria crassissima Caribbean 根據(jù) NCBI數(shù)據(jù)庫登錄的紅藻基因序列 ， 采用 rDNA轉(zhuǎn)錄間隔區(qū) ITS1和 ITS2進行親源關(guān)系的研究 。 實驗原理 技術(shù)路線 設(shè)計引物 ?總 DNA提取或裂解藻細胞?PCR?回收目的片段 ?TA克隆 ?轉(zhuǎn)化 ?測序 ?分析 實驗過程要求同前 真核生物 rDNA結(jié)構(gòu)單元 SSU rDNA and ITS *Curdiea/Melanthalia *Gracilariopsis/Gracilariophila:^Atlantic species *Gracilaria: ^Atlantic cylindrical henriquesiana/^gracilis/^domingensis clades ITS1:Pacific/Atlantic/cylindrical henriquesiana lineage/gracilis lineage/flatted or pressed Atlantic specieslow bootstrap value ? ITS2: tikvahiae lineagepressed to flatted forms with “ textorri” type spermatangia and similar morphology/cervicornis lineagelow bootstrap
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