【文章內(nèi)容簡介】 方法 .........................................................................................................45 基因組 DNA 提取、純化與濃度測定（大量提?。?............................45 AFLP 分析（熒光標記檢測） ................................................................46 AFLP 分析（銀染檢測） ........................................................................50 差異片段回收、克隆 ...............................................................................52 差異片段序列分析及同源性比較 ...........................................................57 AFLPSTS 分子標記的設計與驗證 ........................................................57 結(jié)果與分析 .....................................................................................................58 AFLP 熒光檢測 ........................................................................................58 AFLP 銀染檢測 ........................................................................................60 AFLP 擴增類型 ........................................................................................61 AFLPSTS 分子標記的設計和驗證 ........................................................63 討論 .................................................................................................................65 AFLP 熒光檢測同 AFLP 銀染檢測比較 ................................................65 大麥 2H 染色體導入小麥背景中后的基因組結(jié)構(gòu)變異 ........................66III / 178 AFLP 分子標記轉(zhuǎn)化 STS 分子標記 .......................................................68第 4 章 小麥遺傳背景中大麥 2H 染色體基因組甲基化分析 ...............................75 實驗材料 .........................................................................................................75 實驗方法 .........................................................................................................75 基因組 DNA 提取、純化與濃度測定（大量提?。?............................75 MSAP 分析（熒光標記檢測） ...............................................................75 MSAP 分析（非熒光標記檢測） ...........................................................78 差異片段回收、克隆 ...............................................................................80 差異片段序列分析及同源性比較 ...........................................................80 結(jié)果與分析 .....................................................................................................80 MSAP 熒光檢測 .......................................................................................80 MSAP 擴增類型 .......................................................................................82 MSAP 差異片段回收、測序與同源性分析 ...........................................84 討論 .................................................................................................................86第 5 章 小麥遺傳背景中大麥 2H 染色體基因組表達分析 ...................................91 實驗材料 .........................................................................................................91 實驗方法 .........................................................................................................91 實驗材料處理 ...........................................................................................91 總 RNA 提取 ............................................................................................91 cDNA 第一鏈合成 ...................................................................................92 cDNA 第二條鏈的合成 ...........................................................................93 cDNA 第二條鏈的純化 ...........................................................................93 cDNAAFLP 熒光檢測 ............................................................................94 差異片段序列分析及同源性比較 ...........................................................94 結(jié)果與分析 .....................................................................................................95 cDNAAFLP 熒光檢測 ............................................................................95 cDNAAFLP 差異片段回收與測序 ........................................................98 討論 ...............................................................................................................100第 6 章 小麥遺傳背景中大麥 2H 染色體蛋白質(zhì)組分析 .....................................115 實驗材料 .......................................................................................................115 實驗方法 .......................................................................................................115 品質(zhì)測定 .................................................................................................115 籽粒胚乳超微結(jié)構(gòu)觀察 .........................................................................115 2DPAGE 分析 .......................................................................................115 iTRAQ 分析 ............................................................................................120 結(jié)果與討論 ...................................................................................................123 品質(zhì)測定 .................................................................................................123 籽粒胚乳超微結(jié)構(gòu)觀察 .........................................................................123 籽粒種子總蛋白雙向電泳分析 .............................................................124 籽?？偟鞍?iTRAQ 分析 .......................................................................126第 7 章 結(jié)論 ............................................................................................................131參考文獻 ....................................................................................................................133攻讀學位期間發(fā)表的學術(shù)論文 ................................................................................151致 謝 ....................................................................................................................153I / 178英文縮寫詞英文縮寫 英文全稱 中文全稱及注釋ABAAFLPAmpAbscisic acidAmplified fragment length polymorphismAminobenzyl penicillin脫落酸擴增片段長度多態(tài)性氨芐青霉素BASIBLASTBarley αamylase/subtilisin inhibitorBasic Local Alignment Search Tool大麥 α淀粉酶抑制蛋白基本局部比對搜索工具