慢病毒顆粒制備和應(yīng)用(編輯修改稿)
2025-06-22 12:01 本頁面
【文章內(nèi)容簡介】 Genome No No* Yes Yes Immune Response in Target Cells High Very Low Low Moderate Relative Viral Titer XXXX XXX XXX XX Relative Transduction Efficiency XXXX XXX XXX XX *Native AAV will integrate, but rebinant AAV rarely does. HIV Viron 慢病毒載體（ Lentiviral Vector ）主要是以 HIV1（人類免疫缺陷 I型病毒）為基礎(chǔ)發(fā)展起來的基因轉(zhuǎn)移載體 。 HIV amp。 replication Packaging mix four plasmids system C MV O R Fp U C o r i .G FP P u r oI R E SWPRES V 4 0Amp rU 5 / R SV ( U 3 )DU3/U55’ L T R3’LTRRREcPPT?p Lv R C M V G F P P u r oCMV gag pol RRE poly A EF1α REV poly A CMV poly A VSVG 包膜蛋白質(zhì)粒 包裝質(zhì)粒 + Mammalian Constitutive promoters Qin JY, et al. (2022) Systematic Comparison of Constitutive Promoters and the DoxycyclineInducible Promoter. PLoS ONE 5(5): e10611 Packaging system T a b l e 1 . H o s t c e l l r a n g e o f v i r u s p s e u d o t y p e d w i t h d i f f e r e n t e n v e l o p e p r o t e i n s . G P 70 4070AE n ve lop eE c o t r o p i c A m p h o t r o p i c D u a l t r o p i c a n t r o p i cP Lenti particle Packaging Plate 293T cells for transfection. Prepare plasmid mix + EndoFectin Add to 293T cells Change Medium Harvest medium (Viruses) Lentivirus titration Lentivirus purification and concentration.
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