研究生講課腫瘤干細(xì)胞(編輯修改稿)
2025-05-30 02:30 本頁(yè)面
【文章內(nèi)容簡(jiǎn)介】 PLE DIGESTION: Collagenase I 37186。C 2 hours Washing SINGLE CELL SUSPENSION CULTURING: Serum free medium 原代肺癌細(xì)胞培養(yǎng) 原代培養(yǎng)建立的肺癌細(xì)胞系 We have established 5 squamous carcinoma(B), 4 adenocarcinoma(D), 1 large cell lung cancer(C), 1 small cell lung cancer(A), and 1 lung carcinoid cell lines. 原代培養(yǎng)的肺癌細(xì)胞在 NOD/SCID 小鼠能形成腫瘤 原代培養(yǎng)的肺癌細(xì)胞中含有一小群 CD44 強(qiáng)陽(yáng)性的細(xì)胞 Small cell lung cancer(LC004), large cell lung cancer(LC006) have cd44 strong positive population cells. CD44 強(qiáng)陽(yáng)性細(xì)胞具有較強(qiáng)的增值能力 ? When seeded 500 cells/well, there was no obvious difference between the CD44++ and CD44+/ cells(figure A), but when seeded 150 cells/well, CD44++ cells much stronger proliferative ability(figure B). CD44++ 細(xì)胞具有較強(qiáng)的克隆形成能力 ? A: large cell lung cancer cell line(LC006) ? B: small cell line cell line(LC004) 只有 CD44++的細(xì)胞可以形成 holoclone ? When single cell seeded into 96 well plate, only CD44++CD+ cells could form holoclone, 100 CD44++CD90+ cells could form about 13 holoclones. 只有 CD44++的細(xì)胞在超低粘附的培養(yǎng)板中形成細(xì)胞球 Aamp。C: cell spheres formed by CD44++ cells of LC006 and LC021. Bamp。D: no c
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