細(xì)胞計(jì)數(shù)板使用方法(編輯修改稿)
2025-05-05 01:46 本頁面
【文章內(nèi)容簡(jiǎn)介】 ，無草黃色折光，可隱約見到細(xì)胞核。此外，當(dāng)病人急性嚴(yán)重貧血時(shí)網(wǎng)織紅細(xì)胞可提前大量釋放，也給紅細(xì)胞計(jì)數(shù)帶來一定的干擾，而且影響網(wǎng)織紅細(xì)胞絕對(duì)值計(jì)算結(jié)果。其校正方法有待探討。Using a Counting Chamber For microbiology, cell culture, and many applications that require use of suspensions of cells it is necessary to determine cell concentration. One can often determine cell density of a suspension spectrophotometrically, however that form of determination does not allow an assessment of cell viability, nor can one distinguish cell types. A device used for determining the number of cells per unit volume of a suspension is called a counting chamber. The most widely used type of chamber is called a hemocytometer, since it was originally designed for performing blood cell counts. To prepare the counting chamber the mirrorlike polished surface is carefully cleaned with lens paper. The coverslip is also cleaned. Coverslips for counting chambers are specially made and are thicker than those for conventional microscopy, since they must be heavy enough to overe the surface tension of a drop of liquid. The coverslip is placed over the counting surface prior to putting on the cell suspension. The suspension is introduced into one of the Vshaped wells with a pasteur or other type of pipet. The area under the coverslip fills by capillary action. Enough liquid should be introduced so that the mirrored surface is just covered. The charged counting chamber is then placed on the microscope stage and the counting grid is brought into focus at low power. It is essential to be extremely careful with higher power objectives, since the counting chamber is mu
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