細(xì)胞計數(shù)板使用方法(已改無錯字)
2023-05-09 01:46:46 本頁面
【正文】 ch thicker than a conventional slide. The chamber or an objective lens may be damaged if the user is not not careful. One entire grid on standard hemacytometers with Neubauer rulings can be seen at 40x (4x objective). The main divisions separate the grid into 9 large squares (like a tictactoe grid). Each square has a surface area of one square mm, and the depth of the chamber is mm. Thus the entire counting grid lies under a volume of mmcubed Suspensions should be dilute enough so that the cells or other particles do not overlap each other on the grid, and should be uniformly distributed. To perform the count, determine the magnification needed to recognize the desired cell type. Now systematically count the cells in selected squares so that the total count is 100 cells or so (number of cells needed for a statistically significant count). For large cells this may mean counting the four large corner squares and the middle one. For a dense suspension of small cells you may wish to count the cells in the four 1/25 sq. mm corners plus the middle square in the central square. Always decide on a specific counting patter to avoid bias. For cells that overlap a ruling, count a cell as in if it overlaps the top or right ruling, and out if it overlaps the bottom or left ruling.Here is a way to determine a particle count using a Neubauer hemocytometer. Suppose that you conduct a count as described above, and count 187 particles in the five small squares described. Each square has an area of 1/25 mmsquared (that is, mmsquared) and depth of mm. The total volume in each squa
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