【文章內(nèi)容簡介】 usecTE KD0 secD1 secD9 secD11 secD16 secDELTA secDELTA1 secFACTOR1 4IN0 secl1 24l3 128SCALEF 6============ CHANNEL f1 =============NUC1 1HP1 usecP2 usecP5 usecP6 usecP7 usecP17 usecPL1 dBPL10 dBSFO1 MHz============ GRADIENT CHANNEL ========GPNAM1 GPNAM2 GPX1 %GPX2 %GPY1 %GPY2 %GPZ1 %GPZ2 %P16 usecF1 Acquisition parametersND0 1TD 256SFO1 MHzFIDRES HzSW ppmF2 Processing parametersSI 1024SF MHzWDW QSINESSB 4LB HzGB 0PC F1 Processing parametersSI 1024MC2 echoantiechoSF MHzWDW QSINESSB 4LB HzGB 0H O CH 1HC C CH 2HH 3HH 4HH2 TOCSY (TOtal Correlation SpectroscopY) or HOHAHA(Homonuclear HartmanHahn Spectroscopy) t1 ? ? MLEV17 AQ Pulse sequence for a TOCSY spectrum. H O CH 1HC C CH 2HH 3HH 4HHH O CH 1HC C CH 2HH 3HH 4HHH O CH 1HC C CH 2HH 3HH 4HHCOSY RLCOSY TOCSY 不同的混合時間給出不同接力程度的 TOCSY譜 當混合時間很小時， TOCSY譜相當于 COSY譜 2D Gradient TOCSY, 混合時間 10ms ppm ppmCurrent Data ParametersNAME buttoc10EXPNO 1PROCNO 1F2 Acquisition ParametersDate_ 20221102Time INSTRUM DRX500PROBHD 5 mm TBI 1H/PULPROG mlevetgpTD 1024SOLVENT CDCl3NS 8DS 16SWH HzFIDRES HzAQ secRG 16DW usecDE usecTE KD0 secD1 secD9 secD11 secD16 secDELTA secDELTA1 secFACTOR1 0IN0 secl1 0l3 128SCALEF 6============ CHANNEL f1 =============NUC1 1HP1 usecP2 usecP5 usecP6 usecP7 usecP17 usecPL1 dBPL10 dBSFO1 MHz============ GRADIENT CHANNEL ========GPNAM1 GPNAM2 GPX1 %GPX2 %GPY1 %GPY2 %GPZ1 %GPZ2 %P16 usecF1 Acquisition parametersND0 1TD 256SFO1 MHzFIDRES HzSW ppmFnMODE undefinedF2 Processing parametersSI 1024SF MHzWDW SINESSB 3LB HzGB 0PC F1 Processing parametersSI 1024MC2 echoantiechoSF MHzWDW SINESSB 3LB HzGB 0相當 COSY譜 1 2 3 4 1 3 4 12 23 34 H O CH 1HC C CH 2HH 3HH 4HH2 2D Gradient COSY45 ppm ppmCurrent Data ParametersNAME butcosyEXPNO 1PROCNO 1F2 Acquisition ParametersDate_ 20221102Time INSTRUM DRX500PROBHD 5 mm TBI 1H/PULPROG cosygpTD 1024SOLVENT CDCl3NS 1DS 16SWH HzFIDRES HzAQ secRG DW usecDE usecTE KD0 secD1 secD13 secD16 secIN0 sec============ CHANNEL f1 =============NUC1 1HP0 usecP1 usecPL1 dBSFO1 MHz============ GRADIENT CHANNEL ========GPNAM1 GPNAM2 GPX1 %GPX2 %GPY1 %GPY2 %GPZ1 %GPZ2 %P16 usecF1 Acquisition parametersND0 1TD 256SFO1 MHzFIDRES HzSW ppmFnMODE undefinedF2 Processing parametersSI 2048SF MHzWDW QSINESSB 0LB HzGB 0PC F1 Processing parametersSI 1024MC2 QFSF MHzWDW QSINESSB 0LB HzGB 01 2 3 4 1 3 4 H O CH 1HC C CH 2HH 3HH 4HH12 23 34 2 2D Gradient TOCSY, 混合時間 20ms ppm ppmCurrent Data ParametersNAME buttoc20EXPNO 1PROCNO 1F2 Acquisition ParametersDate_ 20221102Time INSTRUM DRX500PROBHD 5 mm TBI 1H/PULPROG mlevetgpTD 1024SOLVENT CDCl3NS 8DS 16SWH HzFIDRES HzAQ secRG 16DW usecDE