stemcellsandlineagesmechanismsoflung…：干細胞和肺細胞的機制…(編輯修改稿)
2025-02-15 00:10 本頁面
【文章內(nèi)容簡介】 the normal ventral secondary branch (V1) and an ectopic branch (V*) that forms earlier and proximal to V1. V* has already sprouted additional generations of branches. Below, lineage and schematic show V* plus additional ectopic ventral branches (V**, V***。 dashed lines in schematic) seen in other Spry22/2 lungs (Supplementary ). Scale bar (for a and b), 200 mm. Geic control of branching ? Shh (Epith.), Hip1, Ptc1,Gli2, Gli3 (mesen.) ? FGF10, FGF9 (mesen.) ? TTF1 aka Titf1 (Epith.) ? Foxa1+Foxa2, single KOs branch normally req. for Shh expression in epith. ? BMP4 (Tgf? family) (mesen.) ? Pod1 (bHLH TF in mesen.) ? ?catenin signalling in epith. And mesen. ? Foxf1 (mesen.) ? Tbx4, 5 req. for Fgf10 express. in mesen. (siRNA studies) ? Hoxa5 (mesen.) ? Spry2 FGF tyrosine kinase inhibitor (Epith.) Geic control of branching Reciprocal EpithelialMesenchymal interactions mediated by growth factor and cell contactregulated transcriptional works. These interactions both constitute a program of growth, morphogenesis and differentiation and then help establish the quazistable epigeic states that characterize each differentiated cell. Review of mouse lung development Neonatology: SE Wert 5th edition 1999. Cordoso, Dev. Dyn. 219:121 (2022) Pseudoglandular Canalicular Saccular Alveolar Overview of mouse lung development Canalicular and saccular phases (later maturation ) ? Transition from undifferentiated to fully differentiated cells and marked reduction in cell proliferation. ? KOs, conditional KOs and conditional transgenics ? In vitro culturing of lungs Canalicular phase () what cell types are formed in conducting airways? p63 KO eliminates basal cells Foxj1 KO eliminates cilia on cells Gfi1 KO reduces number of PNECs Early Saccular ph
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