【文章內(nèi)容簡介】 3 種真菌培養(yǎng)時(shí)間、發(fā)酵液添加時(shí)間及添加量對 N5 菌株納他霉素產(chǎn)量的影響，以促進(jìn)菌株納他霉素的合成。實(shí)驗(yàn)結(jié)果表明這三種真菌的發(fā)酵液均對納塔爾鏈霉菌 N5菌株的納他霉素產(chǎn)量有明顯促進(jìn)作用。產(chǎn)黃青霉和黑曲霉培養(yǎng) 1d時(shí)，其發(fā)酵液對 N5菌株納他霉素的合成促進(jìn)效果最佳，而釀酒酵母在 3d時(shí)促進(jìn)效果最好。三種真菌最佳發(fā)酵液添加時(shí)間及添加量均為 12h 和 5％。具體這些真菌發(fā)酵 液中何種因素對 N5 菌株納他霉素生產(chǎn)起到促進(jìn)作用還不清楚，有待于進(jìn)一步實(shí)驗(yàn)研究 。 參考文獻(xiàn) [1] Marta V Mendes ， sedef Tunca ， Nuria Anton et al The twoponent phoRphoP system of streptomyces natalensis 。inactivation or deletion of phoP reduces the negative phosphate regulation of pimaricin biosynthesis metabolic engineering 2021 ,9 (2)： 217— 227； [2] PEDERSEN J C Natamycin as a fungicide imager media ［ J］ Applied and environment 。 microbiobogy 10 1992 ， 58（ 3） .1064— 1066； [3] 陳曉麗、呂振岳、黃東東等。新型天然食品防腐劑納他霉素的研究進(jìn)展［ J］食品研究與開發(fā) .（ 4）： 23； [4] 盧國營、張朝輝、洪偉杰，新型防腐劑納他霉素的研究進(jìn)展［ J］中國食品添加劑 2021（ 3）： 30— 32； [5] 魏寶東、孟寬軍、天然生物性食品防腐劑納他霉素的特性及應(yīng)用［ J］，遼寧農(nóng)業(yè)科學(xué) 2021 （ 2）： 24— 26； [6] 賀家亮、張敏、趙勝娟，納他霉素的研究現(xiàn)狀［ J］中國食品添加劑 2021（ 3）： 30— 32； [7] Martin J F DEMAIN AL control of antibiotic biosynthesis 1980（ 02）； [8] 葉輝、王兆慧、陳佩林等 微生物誘導(dǎo)子對卡諾氏菌屬 N05205 菌株的影響［ J］，生物技術(shù)， 2021 14（ 6）：57— 58； [9] 邊猛、田黎、張久明等，誘導(dǎo) 子對海洋真菌 penicillium crustosum 204— 215 菌株生物堿代謝的調(diào)節(jié)作用［ J］海洋學(xué)報(bào)， （ 2）： 117— 123； [10] 邊猛 海洋真菌產(chǎn)生生物堿代謝調(diào)節(jié)的研究［ D］：［碩士學(xué)位論文］青島科技大學(xué) 生物化工系 。 [11] 史強(qiáng) 真菌代謝產(chǎn)物中促進(jìn)納他霉素合成的誘導(dǎo)子的研究［ D］：［碩士學(xué)位論文］江南大學(xué)。 致謝 感謝曹老師對于本試驗(yàn)的悉心指導(dǎo)，她 對生物學(xué)科領(lǐng)域中的基本問題和 發(fā)展前景 的敏銳洞察力，對學(xué)術(shù)思想的重視，對選題和研究內(nèi)容的嚴(yán)格要求，以及對論文工作中遇到的關(guān)鍵問題和疑難問題的指 導(dǎo)使我受益匪淺。 她對試驗(yàn)的研究態(tài)度和嚴(yán)謹(jǐn)?shù)墓ぷ髯黠L(fēng)給予我今后的學(xué)習(xí)和工作很深的啟示。同時(shí)我還要感謝一起做實(shí)驗(yàn)的同學(xué)，他們在我的實(shí)驗(yàn)過程中給了我很大的幫助。 The effect of fungus fermented liquid on the output of Streptomyces natalensis natamycin Author: instructor: (2021 College of life science of Anhui agricultural Universitylevel biotechnology class 1, 09121012,) Abstract: Natamycin is a broadspectrum antifungal agent generated by the fermentation of the streptomyces natalensis,streptomyces gilvosporeus,streptomyces chattanoogensis and other can be used as biological preservatives because it has low toxicity and change food taste experiment adopts the methods of adding Fungal fermentation broth to streptomyces natalensis N5 strains to improve natamycin production of the results reveal that Fermentation broth of Penicillium chrysogenum, Aspergillus niger, Saccharomyces cerevisiae can all improve Production of natamycin has the best effect on the improvement of the synthesis of natamycin when Penicillium chrysogenum and Aspergillus niger are cultured for one ,Saccharomyces cerevisiae has the best effect of improvement when culture for three days,and when the production of natamycin is one time higher than samples without treatment of fungal fermentation broth. Key words: natamycin productivity Penicillium chrysogenum Aspergillus niger Saccharomyces cerevisiae ag an employment tribunal clai Emloyment tribunals sort out disagreements between employers and employees. You may need to make a claim to an employment tribunal if: you don39。t 11 agree with the disciplinary action your employer has taken against you your employer dismisses you and you think that you have been dismissed unfairly. For more informu, take advice from one of the anisations listed under Further help. Employment tribunals are less formal than some other courts, but it is still a legal process and you will need to give evidence under an oath or affirmation. Most people find making a claim to an employment tribunal challenging. If you are thinking about making a claim to an employment tribunal, you should get help straight away from one of the anisations listed under Further help. ation about dismissal and unfair dismissal, see Dismissal. You can make a claim to an employment tribunal, even if you haven39。t appealed against the disciplinary action your employer has taken against you. However, if you win your case, the tribunal may reduce any pensation awarded to you as a result of your failure to appeal. Remember that in most cases you must make an application to an employment tribunal within three months of the date when the event you are plaining about happened. If your application is received after this time limit, the tribunal will not usually accept i. If you are worried about how the time limits apply to you If you are being represented by a solicitor at the tribunal, they may ask you to sign an agreement where you pay their fee out of your pensation if you win the case. This is known as a damagesbased agreement. In England and Wales, your solicitor can39。t charge you more than 35% of your pensation if you win the case. you39。re clea