【正文】 因排列順序的相同（共線性）或相似性（同線性），并由此對相關(guān)物種的基因組結(jié)構(gòu)和起源進(jìn)化進(jìn)行分析。, De225。rez L, 2000. Genetic linkage map of the edible basidiomycete Pleurotus ostreatus. Applied and Environmental Microbiology, 66(12): 52905300 , , , Ritter E, Pisabarro AG, Ram237。 V, Le Tacon F, Marmeisse R, Melayah D, Montanini B, Muratet M, Nehls U, NiculitaHirzel H, OudotLe Secq MP, Peter M, Quesneville H, Rajashekar B, Reich M, Rouhier N, Schmutz J, Yin T, Chalot M, Henrissat B, Kues U, Lucas S, van de Peer Y, Podila GK, Polle A, Pukkila PJ, Richardson PM, Rouz233。rkBaydar N, Akkurt M, Maul E, Eibach R, T246。rez L, , 2006. Genetic linkage map and expression analysis of genes expressed in the lamellae of the edible basidiomycete Pleurotus ostreatus. Fungal Genetics and Biology, 43: 376387Peever TL, Canihos Y, Olsen L, Iba241。nchez G, Zapater MF, LunaMart237。s B, Kiss P, Jakab J, Kiss GB, 2007. Towards the map based cloning of a recessive Fusarium resistance determinant from diploid Medicago sativa. Acta Phytopathologica et Entomologica Hungarica, 42(2): 279289, , , , 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3): 314331Bowden CG, Royse D, May B, 1991. Linkage relationships of allozymeencoding loci in shiitake Lentinula edodes. Genmoe, 34: 652657Callac C, Desmerger RW, Kerrigan RW, Imbernon M, 1997. Conservation of genetic linkage with map expansion in distantly related crosses of Agaricus bisporus. FEMS Microbiology Letters, 146: 235240Chang ST, Miles PG, 1989. Edible mushrooms and their cultivation. CRC Press, Boca Raton, FL. 15Clark TA, Anderson JB, 2004. Dikaryons of the basidiomycete fungus Schizophyllum mune: evolution in longterm culture. Genetics, 167: 16631675Darvasi A, Weinreb A, Minke V, Weller JI, Soller M, 1993. Detecting markerQTL linkage and estimating QTL gene effect and map location using a saturated genetic map. Genetics, 134: 943951de Vos L, Myburg AA, Wingfield MJ, Desjardins AE, Gordon TR, Wingfield BD, 2007. Complete genetic linkage maps from an interspecific cross between Fusarium circinatum and Fusarium subglutinans. Fungal Genetics and Biology, 44: 701714Debry RW, Seldin MF, 1996. Human mouse homology relationships. Genomics, 33: 337351Dunford RP, Kurata N, Laurie DA, Money TA, Minobe Y, Moore G, 1995. Conservation of finescale DNA marker order in the genomes of rice and Triticeae. Nucleic Acids Research, 23: 27242728Elliott TJ, 1985. The genetics and breeding of species of Agaricus. In: Flegg PB, Spencer DM, Wood DA (eds.) The biology and technology of the cultivated mushroom. John Wiley amp。目前，依據(jù)遺傳連鎖圖譜進(jìn)行比較基因組學(xué)的研究已經(jīng)在高等動(dòng)植物之間陸續(xù)展開（Dunford et al. 1995；Debry amp。Park et al.（2006）以Larraya et al.（2000）構(gòu)建糙皮側(cè)耳遺傳圖譜時(shí)采用的作圖群體及其親本為材料，鑒定并定位了82個(gè)在菌褶中表達(dá)的功能基因，進(jìn)一步豐富了該遺傳圖譜，這些工作為研究從營養(yǎng)生長到生殖生長轉(zhuǎn)變過程中的基因調(diào)控提供了試驗(yàn)平臺；Labb233。3 大型真菌分子遺傳圖譜研究的方向與前景 高質(zhì)量遺傳連鎖圖譜的構(gòu)建遺傳連鎖圖譜飽和度是指單位長度染色體上已定位的標(biāo)記數(shù)或標(biāo)記在染色體上的密度。 Sunnerhagen 2006）的研究。該圖譜包含10個(gè)功能性基因位點(diǎn)，這對于香菇遺傳連鎖圖譜之間的對比分析較為有利，如該圖中MatA和PriA都定位在連鎖群LGⅡ上，表明此連鎖群與Kwan構(gòu)建的連鎖圖中LG Ⅲ相對應(yīng)（Kwan amp。 Takemaru 1975）。該圖跨度28cM，有4個(gè)源自RFLP的SCAR標(biāo)記和一個(gè)異型酶羧肽酶位點(diǎn)PEP2，一個(gè)交配型座位MAT以及擔(dān)孢子數(shù)量位點(diǎn)BSN。表1 大型真菌遺傳連鎖圖譜構(gòu)建情況Table 1 Construction of macrofungi genetic linkage map物種Species構(gòu)圖群體Population遺傳標(biāo)記Genetic marker連鎖圖譜Genetic linkage map參考文獻(xiàn)Reference標(biāo)記數(shù)Number覆蓋長度Length (cM)連鎖群數(shù)Linkage number平均圖距Average distance (cM)雙孢蘑菇Agaricus bisporus52RAPD、RFLP6411/Kerrigan et al. 1993103SCAR、Others7281/Callac et al. 1997121RAPD、SCAR265/Moquet et al. 1999118AFLP、SSR、CAPS、Others320115613Marie et al. 2010雙色蠟?zāi)accaria bicolor45AFLP、RAPD、SSR、SNP29481213Labb233。1 大型真菌分子遺傳圖譜研究的現(xiàn)狀真菌遺傳圖譜的構(gòu)建工作較高等動(dòng)植物起步晚，研究基礎(chǔ)相對落后，其