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此刻,天空是陰暗的,空氣里有著剛下過雨之后的清新因子。想要的,不想要的,界限明確,好像沒有什么可以撼動(dòng)自己。 Junior high school, thought to have a crush on just means that the real growth, but over the past three years later, his writing of alumni in peace, suddenly found that isn39。s governing body, has also ordered an immediate investigation into the referee39。t appealed against the disciplinary action your employer has taken against you. However, if you win your case, the tribunal may reduce any pensation awarded to you as a result of your failure to appeal. Remember that in most cases you must make an application to an employment tribunal within three months of the date when the event you are plaining about happened. If your application is received after this time limit, the tribunal will not usually accept it. If you are worried about how the time limits apply to you, take advice from one of the anisations listed under Further help. Employment tribunals are less formal than some other courts, but it is still a legal process and you will need to give evidence under an oath or affirmation. Most people find making a claim to an employment tribunal challenging. If you are thinking about making a claim to an employment tribunal, you should get help straight away from one of the anisations listed under Further help. If you are being represented by a solicitor at the tribunal, they may ask you to sign an agreement where you pay their fee out of your pensation if you win the case. This is known as a damagesbased agreement. In England and Wales, your solicitor can39。卞老師嚴(yán)謹(jǐn)?shù)闹螌W(xué)態(tài)度、淵博的專業(yè)知識(shí)、敏銳的學(xué)術(shù)眼光、精益求精的精神給我留下了深刻的印象,并對(duì)我的學(xué)習(xí)和工作產(chǎn)生極大地促進(jìn)作用。 鈣離子濃度(ug/ml) 菌落平均直徑長度( cm) 3d 6d 9d 12d 15d 對(duì)照組 300 3 350 400 450 500 11 首先,本次實(shí)驗(yàn),只做了單因素影響的實(shí)驗(yàn),也就是說只研究了單一金屬離子對(duì)杏鮑菇菌絲在 PDA培養(yǎng)基中生長的影響,當(dāng)兩種金屬離子同時(shí)加入 PDA培養(yǎng)基時(shí),其作用效果如何,還有待進(jìn)一步研究。 觀察記錄, 定時(shí)觀測菌絲體的生長情況,記錄菌落直徑累計(jì)長度,并填寫下列表格,同時(shí)觀察菌絲濃密和菌落形態(tài)。 培養(yǎng)皿編號(hào) 菌落直徑長度( cm) 3d 6d 9d 12d 15d 7 8 9 平均 培養(yǎng)皿編號(hào) 菌落直徑長度( cm) 3d 6d 9d 12d 15d 1 2 3 平均 培養(yǎng)皿編號(hào) 菌落直徑長度( cm) 3d 6d 9d 12d 15d 4 5 6 平均 B 1100ug/ml C 1150ug/ml A 對(duì)照組 表組 1 鐵離子對(duì)杏鮑菇菌絲生長的影響 6 培養(yǎng)皿編號(hào) 菌落直徑長度( cm) 3d 6d 9d 12d 15d 15 17 18 平均 鈣元素對(duì)杏鮑菇菌絲生長的影響 配制 鈣元素母液:精確稱量氯化鈣 1g, 用熱水溶解,然后倒入 1000ml 容量瓶中,定容至 1000ml 為 1mg/ml 的鈣元素母液; 不同濃度鈣元素溶液的的配制:用移液管從 1mg/ml 的氯化鈣水溶液中分別取3ml,4ml,5ml 于貼好標(biāo)簽的 100ml 的容量瓶中,分別定容至 100ml,得到300ug/ml , 350ug/ml, 400ug/ml, 450ug/ml, 500ug/ml[15] 5 個(gè)濃度的鈣元素溶液。然后趁熱分裝入試管,每管大約 15ml,每個(gè)濃度裝 3 支試管。選擇生長良好,生物特征明顯,無雜菌的菌種用于接下來的實(shí)驗(yàn) [12]。 2 實(shí)驗(yàn)部分 3 主要儀器與試劑 主要儀器 立式壓力蒸汽滅菌器 上海博迅實(shí)業(yè) 有限公司醫(yī)療設(shè)備廠 ,220V 無菌操作工作臺(tái) 上海亞榮生化儀器廠 電子萬用爐 北京市永光明醫(yī)療儀器廠 DL1 型 ,1000W,220V 電子分析天平 中國輕工業(yè)機(jī)器總公司常熟衡天 DF110 型 干燥箱 上海市實(shí)驗(yàn)儀器總廠 2022 型 ,220V,300℃ 恒溫生化培養(yǎng)箱 上海綠宇生物科技有限公司 RXZ300D 型 220V 0~ 50℃ 基本試劑和藥品 氯化鐵 分析純 ,含量大于 99% 北京化學(xué)試劑公司 無水氯化鈣 分析純, 含量大于 99% 北京化工廠 葡萄糖 分析純 , 含量大于 % 天津市永大化學(xué)試劑開發(fā)中心 瓊脂 上?;瘜W(xué)試劑站分裝廠 馬鈴薯 郴州市場購買 主要的玻璃儀器及其他實(shí)驗(yàn)用具 燒杯 ,試管 ,玻璃棒 ,容量瓶( 1000ml, 100ml) ,錐形瓶 ,移液管( 100ml, 10ml) ,漏斗 ,紗布 ,接種針 ,酒精燈 ,標(biāo)簽紙 ,培養(yǎng)皿 ,棉花塞 ,牛皮紙,