【正文】 lk emulsion. Mix intimately but gently, and pour into plates. (Prepare the eggyolk emulsion by disinfecting the surface of whole shell eggs, aseptically cracking the eggs, and separating out intact yolks into a sterile graduated cylinder. Add sterile saline TS to obtain a 3to 7ratio of egg yolk to saline. Add to a sterile blender cup, and mix at high speed for 5seconds.) pHafter sterilization:177。. –Johnson Agar Medium Pancreatic Digest of Casein Yeast Extract Mannitol Dibasic Potassium Phosphate Lithium Chloride Glycine Agar Phenol Red Water 1000mL Boil the solution of solids for ,cool to between 45 and 50 ,and add 20mLof sterile potassium tellurite solution (1in 100). pHafter sterilization:177。. Agar Medium Pancreatic Digest of Gelatin Magnesium Chloride Potassium Sulfate Agar Cetyl Trimethylammonium Bromide (Cetrimide) Glycerin Water 1000mL Dissolve all solid ponents in the water, and add the glycerin. Heat,with frequent agitation, and boil for 1minute to effect solution. pHafter sterilization:177。. VIII. Pseudomonas Agar Medium for Detection of Fluorescin Pancreatic Digest of Casein Peptic Digest of Animal Tissue Anhydrous Dibasic Potassium Phosphate Magnesium Sulfate (MgSO47H2O) Glycerin Agar Water 1000mL Dissolve the solid ponents in the water before adding the ,with frequent agitation,and boil for 1minute to effect solution. pHafter sterilization:177。. Agar Medium for Detection of Pyocyanin Pancreatic Digest of Gelatin Anhydrous Magnesium Chloride Anhydrous Potassium Sulfate Agar Glycerin Water 1000mL Dissolve the solid ponents in the water before adding the ,with frequent agitation,and boil for 1minute to effect solution. pHafter sterilization:177。. Lactose Medium Beef Extract Pancreatic Digest of Gelatin Lactose Water 1000mL Cool as quickly as possible after sterilization. pHafter sterilization:177。. Selenite–Cystine Medium Pancreatic Digest of Casein Lactose Sodium Phosphate Sodium Acid Selenite LCystine Water 1000mL Final pH:177。. Mix,and heat to effect in flowing steam for not sterilize. Tetrathionate Medium Pancreatic Digest of Casein Peptic Digest of Animal Tissue Bile Salts Calcium Carbonate Sodium Thiosulfate Water 1000mL Heat the solution of solids to boiling. On the day of use, add a solution prepared by dissolving 5g of potassium iodide and 6g of iodine in 20mLof water. Then add 10mLof a solution of brilliant green (1in 1000),and not heat the medium after adding the brilliant green solution. Green Agar Medium Yeast Extract Peptic Digest of Animal Tissue Pancreatic Digest of Casein Lactose Sodium Chloride Sucrose Phenol Red 80mg Agar Brilliant Green Water 1000mL Boil the solution of solids for just prior to use, melt the medium, pour into petri dishes, and allow to cool. pHafter sterilization:177。. XIV. Xylose–Lysine–Desoxycholate Agar Medium Xylose LLysine Lactose Sucrose Sodium Chloride Yeast Extract Phenol Red 80mg Agar Sodium Desoxycholate Sodium Thiosulfate Ferric Ammonium Citrate 800mg Water 1000mL Final pH:177。. Heat the mixture of solids and water, with swirling, just to the boiling point. Do not overheat or sterilize. Transfer at once to a water bath maintained at about 50 ,and pour into plates as soon as the medium has cooled. Sulfite Agar Medium Beef Extract Pancreatic Digest of Casein Peptic Digest of Animal Tissue Dextrose Sodium Phosphate Ferrous Sulfate 300mg Bismuth Sulfite Indicator Agar Brilliant Green 25mg Water 1000mL Final pH:177。. Heat the mixture of solids and water,with swirling,just to the boiling not overheat or at once to a water bath maintained at about 50 ,and pour into plates as soon as the medium has cooled. Sugar–Iron–Agar Medium Pancreatic Digest of Casein Pancreatic Digest of Animal Tissue Lactose Sucrose Dextrose Ferrous Ammonium Sulfate 200mg Sodium Chloride Sodium Thiosulfate 200mg Agar Phenol Red 25mg Water 1000mL pHafter sterilization:177。. Agar Medium Pancreatic Digest of Gelatin Pancreatic Digest of Casein Peptic Digest of Animal Tissue Lactose Bile Salts Mixture Sodium Chloride Agar Neutral Red 30mg Crystal Violet Water 1000mL Boil the mixture of solids and water for 1minute to effect solution. pHafter sterilization:177。. Eosin–Methylene Blue Agar Medium Pancreatic Digest of Gelatin Dibasic Potassium Phosphate Agar Lactose Eosin Y 400mg Methylene Blue 65mg Water 1000mL Dissolve the pancreatic digest of gelatin, the dibasic potassium phosphate, and the agar in the water, with warming, and allow to cool. Just prior to use, liquefy the gelled agar solution, add the remaining ingredients, as solutions, in the following amounts, and mix: for each 100mLof the liquefied agar solution—5mLof lactose solution (1in 5),2mLof the eosin Ysolution (1in 50),and 2mLof methylene blue solution (1in 300).The finished medium may not be clear. pHafter sterilization:177。. XIX. Sabouraud Dextrose Agar Medium Dextrose 40g Mixture of equal parts of Peptic Digest of Animal Tissue and 10g Pancreatic Digest of Casein Agar 15g Water 1000mL Mix,and boil to effect solution. pHafter sterilization:177。. Dextrose Agar Medium Cook 300g of peeled and diced potatoes in 500mLof water prepared by distillation, filter through cheesecloth, add water prepared by distillation to make 1000mL,and add the following: Agar 15g Glucose 20g Dissolve by heating,and sterilize. pHafter sterilization:177。. For use, just prior to pouring the plates, adjust the melted and cooled to 45medium with sterile tartaric acid solution (1in 10)to a pHof 177。 not reheat the . SAMPLING Provide separate 10mLor 10g specimen