embryoidbodiesfromembryonicstemcellsin-vitro：胚狀體從胚胎干細胞體外培養(yǎng)-在線瀏覽
2025-03-01 07:57本頁面
【正文】 during development ?Ectodermal tissues ?Mesodermal tissues ?Endodermal tissues ? EBs resemble the embryo of the egg cylinder stage ?Neuronal cells ?Cardiac muscle cells ?Hematopoietic cells ?Yolk sac cells ? ES cells have full developmental potential ? EBs from ES cells ? EBs consist of: ? Later stages EBs are posed of: ?Past studies showed specific changes in expression of endoderm marker genes during EB development and suggested that EB formation could be considered an invitro model for endoderm differentiation. ?RNA blot ?Reverse transcription polymerase chain reaction (RTPCR) ?Insitu hybridization ?The nature of endoderm differentiation invitro is discussed in relation to normal embryonic and extraembryonic development invivo. ?This paper extended their analysis and systematically characterized temporal expression patterns of endoderm marker genes during EB formation. Materials and Methods Cell cultures ?ES cell line, D3, cultured ?Incubated 3 days in Du
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