【正文】 y. Purple sweet potatoes are so facile and cheap that they can meet the expectation of industry. There are great potential applications of anthocyanins from purple sweet potato in food pharmaceutical and cosmetic industries. With the salttolerant purple sweet potatoes as raw material, the anthocyanins were extracted via conventional solvent method and ultrasonicassisted method respectively, the effect of ultrosonicassistant treatment was studied under the optimal conditions of solvent method. To obtain a high content of anthocyanins and better quality products, macroporous resins were used in purple sweet potato pigments purification. Reversedphase HPLC with electrospray ionization mass spectrometry were also used to rapidly identify the main anthocyaninsins in indifferent varieties of purple sweet potato, extracts with different methods and eluent with gradientelution. At last, the heat stability and antioxidant activity was studied. Details are concluded as follows:(1) Spectrometry analysis of HPLCDADESI/MS for crude extracts showed that most of anthocyanins in salttolerant purple sweet potato are cyanin and peonin acylated by caffeic acid, ferulic acid, phydroxybenzoic acid and other aromatic acid. Purple sweet potato Z103 has 15 constituents of anthocyanins, of which seven cyanin and eight peonin, one glucoside and the other are diglucosidase, three nonacylated, six acylated and six diacylated anthocyanins, acyl were caffeic acid, ferulic acid, phydroxybenzoic. There was no significant difference in the ponents in different purple sweet potato varieties Z103, Z109, Z109, but the contents are different. (2) The results showed that under the condition of pH , ethanol concentration 20% (volume fraction), liquidsolid ratio 1:30 (g:mL), extraction temperature 50℃, and extraction time 120 min, the extraction rate of anthocyanins was % with conventional solvent method , the content of starch in the residue was g/100g and the loss ratio was %, which showed the method was conducive to the recovery of starch. (3) The effect of ultrosonicassistant treatment was studied under the optimal conditions mentioned above, a higher extraction rate of % was obtained while treated with an ultrasonic power of 90 W. The anthocyanins content of purple sweet potato was mg/100g（db）calculated by the pH differential method. Here, the content of starch in the residue was g/100g and the loss ratio was %. Constituent analysis by HPLCMS also showed that there was no significant difference in the ponents between the extract obtained by conventional solvent method and that by ultrasonicassisted methods. So, the ultrasonicassisted method with a higher extraction rate almost had no impact on position of anthocyanins.(4) The characteristics of static adsorption dynamics and desorption of ten kinds of macroporous resins (HPD300、HPD700、ABHPD400、HPD100A、XNKASD3520and NKAⅡ) used in purple sweet potato pigments purification were investigated. It was indicated that HPD300 was a preferable macroporous resins with the absorption equilibrium rate contant of min1, the absorption equilibrium time is 68 h, and desorption equilibrium time is 12 h. Static absorption thermodynamics reach of HPD300 showed that absorption equation fits the Langmuir equation well at all temperatures studied, the adsorption follow the law of monolayer adsorption and was exothermic. The maximum adsorption of HPD300 to purple sweet potato anthocyanins was mg）的清除能力都隨著濃度的增加而增加，當(dāng)濃度為30 μg/mL時(shí)，%，不同抗氧化劑對(duì)HO自由基清除能力依次為：紫甘薯花色苷茶多酚VE紫甘藍(lán)紅色素， mg/mL、 mg/mL、 mg/ mg/mL；相同濃度下（4 mg/mL），抗氧化劑清除超氧陰離子自由基（O2ˉ（6） 紫甘薯花色苷的熱降解遵循一級(jí)反應(yīng)的動(dòng)力學(xué)規(guī)律， kJ/mol，隨著溫度升高紫甘薯花色苷色素半衰期減小，其熱降解速率隨溫度升高而加快，在25℃下，105 min1， h，100℃下，102 min1， h?；ㄉ战M分在大孔吸附樹(shù)脂HPD300上的吸附行為為放熱過(guò)程，低溫有利于吸附容量的提高。在所研究的濃度范圍內(nèi)，該樹(shù)脂對(duì)紫甘薯花色苷的吸附為單分子層吸附， mg結(jié)果表明，超聲輔助浸提對(duì)產(chǎn)品中花色苷組成基本沒(méi)有影響；由此得出，利用超聲輔助法提取耐鹽紫甘薯的花色苷，提取率較常規(guī)溶劑提取法有較大提高，且對(duì)花色苷組分基本不產(chǎn)生影響。（3） 在上述優(yōu)化條件下，結(jié)合超聲場(chǎng)強(qiáng)化技術(shù)，在超聲功率為90W時(shí)， mg/100 g（db），提取收率一次提取？要跟溶劑提取的結(jié)果對(duì)應(yīng)。主要研究?jī)?nèi)容如下：（1） 紫甘薯花色苷粗提液的HPLCDADESI/MS圖譜分析表明，紫甘薯花色苷主要為被咖啡酸、阿魏酸、對(duì)羥基苯甲酸等芳香酸?；氖杠嚲哲蘸蜕炙庈眨蛔细适鞿103含有15種具有花色苷特征的組分，其中有7種是以矢車菊花色素為苷元，8種是以芍藥花色素為苷元；單糖苷的有1種，其他為雙糖苷，形成花色苷的糖苷為葡萄糖苷和槐糖苷；非?；幕ㄉ沼?種，單?；挠?種，雙?；挠?種，?；鶠榭Х人帷⑽核?、對(duì)羥基苯甲酸；紫甘薯Z10Z110和Z103三個(gè)品種所含花色苷的種類基本相同，含量不同。并利用HPLCDADESI/MS技術(shù)對(duì)不同品種，不同提取方法以及不同洗脫梯度的紫甘薯花色苷提取液進(jìn)行了組分分析。本文以鹽城產(chǎn)的耐鹽紫甘薯為原料，分別采用常規(guī)溶劑提取和超聲輔助溶劑提取法提取其中的花色苷，在優(yōu)化醇水溶劑提取條件的基礎(chǔ)上，進(jìn)一步考察超聲場(chǎng)強(qiáng)化對(duì)提取率的影響，以確定最佳提取工藝。 耐鹽紫甘薯花色苷的提取純化與組分分析及其抗氧化性研究 南 京 工 業(yè) 大 學(xué)學(xué) 位 論 文耐鹽紫甘薯花色苷的提取純化與組分分析及其抗氧化性研究Extraction, Composition Analysis and Antioxidant Activity of Anthocyanins from Salttolerant Purple Sweet Potato摘要耐鹽紫甘薯（Salttolerant purple sweet potato, Ipomoes batatas L.）是適合在沿海灘涂生長(zhǎng)的旋花科一年生草本植物，其所含花色苷是一類優(yōu)質(zhì)的酰基化天然色素，具有著色能力、水溶性和穩(wěn)定性好的特點(diǎn)，對(duì)人類的腫瘤、衰老、心血管病等疾病的預(yù)防與治療有重要的意義。耐鹽紫甘薯原料易得、價(jià)格低廉，能夠適應(yīng)工業(yè)化生產(chǎn)的需要，紫甘薯花色苷在食品、醫(yī)藥及化妝品等領(lǐng)域具有很大的應(yīng)用前景。為得到含量較高、品質(zhì)更好的花色苷產(chǎn)品，本文通過(guò)大孔吸附樹(shù)脂的篩選和吸附性能研究，對(duì)提取液進(jìn)行純化精制。最后，考察了紫甘薯花色苷的熱穩(wěn)定性和抗氧化性。 （2） 紫甘薯花色苷溶劑提取的最佳工藝只能算提取“條件”為：以pH ，體積分?jǐn)?shù)為20%的鹽酸酸化乙醇為提取液、料液比為1:30（g:mL）、浸提溫度為50℃、浸提時(shí)間為120 min，%； g/100g，%，有利于淀粉的回收利用。%； g/100g，%；對(duì)不同提取方法所得的提取液進(jìn)行LCMS分析比較。結(jié)論合并（4） 通過(guò)對(duì)HPD300、HPD700、ABHPD400、HPD100A、XNKASD3520以及NKAⅡ10種大孔吸附樹(shù)脂的靜態(tài)吸附和解吸附實(shí)驗(yàn)篩選，初步選定5種吸附量大、選擇性強(qiáng)、易解吸的樹(shù)脂：HPD300、HPD400、HPD700、XAB8進(jìn)一步進(jìn)行吸附動(dòng)力學(xué)曲線及吸附速率方程和解吸附動(dòng)力學(xué)曲線的研究，確定吸附速率常數(shù)（ min1）較大的HPD300作為吸附劑吸附紫甘薯花色苷，其吸附達(dá)平衡的時(shí)間為68 h，解吸附平衡時(shí)間為12 h；對(duì)HPD300大孔樹(shù)脂的靜態(tài)吸附熱力學(xué)研究，結(jié)果表明，Langmuir方程對(duì)紫甘薯花色苷在HPD300樹(shù)脂上的吸附情況擬合效果較好。g1。（5） 固定床連續(xù)吸附洗脫工藝研究中，對(duì)HPD300樹(shù)脂對(duì)紫甘薯花色苷的吸附和解吸附條件優(yōu)選：，初始濃度C0= mg/ml的花色苷溶液， mL/min，體積為145 mL時(shí)，HPD300樹(shù)脂動(dòng)態(tài)吸附量為： mg/g(樹(shù)脂)，達(dá)到最佳吸附能力；解吸時(shí)，采用80%（v/v）乙醇溶液作為洗脫劑， mL/min，用3倍柱體積洗脫花色苷，%； mg/g，；色價(jià)E1%1cm（528nm）=，；紫甘薯Z103花色苷不同梯度的乙醇洗脫液中，20%乙醇洗脫液因洗脫的花色苷含量微小，檢測(cè)不出花色苷，40%的乙醇洗脫液中含有15種花色苷，60%、（包括2種類型花色苷），80%，說(shuō)明40%的乙醇可以把吸附在樹(shù)脂上的紫甘薯花色苷基本洗脫下來(lái)。（7） 在四個(gè)體外抗氧化性體系研究中，脂質(zhì)體體系， mg/mL的紫甘薯花色苷（%）與同等什么意思？紫甘藍(lán)紅色素（%）的抗氧化能力相近，且高于茶多酚（%）與維生素E（%）；各抗氧化劑對(duì)DPPH）的能力（清除率）大小順序?yàn)樽细适砘ㄉ眨?）＞茶多酚（%）＞紫甘藍(lán)紅色素（%)＞VE（%）， mg/mL、 mg/mL、 mg/ mg/mL；紫甘薯花色苷對(duì)羥自由基（HO的清除能力紫甘薯花色苷＞紫甘藍(lán)紅色素＞茶多酚＞VE， μg/mL、 mg/mL、 mg/ mg/mL。g1.(5) In the dynamic adsorpti