疫學(xué)常用實(shí)驗(yàn)方法(2)-展示頁
2025-05-10 06:20本頁面
【正文】 平 (例如，用放射免疫測定法或酶免疫測定法測定 HBsAg，其敏感度可達(dá) /ml) ELISA酶聯(lián)免疫吸附試驗(yàn) Immunohistochemistry 免疫組織化學(xué) FACS流式細(xì)胞術(shù) Western Blotting免疫印跡技術(shù) Often used methods 1. ELISA(酶聯(lián)免疫吸附試驗(yàn)Enzyme Linked ImmunoSorbent Assay) RIA (radioimmunoassay, Bersonamp。Ab 解離后的抗原或抗體均能保持原有的結(jié)構(gòu)和活性。Commonly used immunological methods Zhihai Qin Institute of Biophysics Chinese Academy of Sciences ?AgAb reation: ELISA, Immunohistochemistry, FACS, Western blot, Hybridoma amp。 monoclonal antibody ?Cell function: proliferation 3H－ TdR MTT CFSE Cytotoxicity 51Cr ?Animal experiment 非共價鍵結(jié)合 I. AntigenAntibody Reaction 抗體的互補(bǔ)決定區(qū) (CDRs) 抗原決定簇 (antigenic determinant) 又稱表位 (epitope) 非共價鍵結(jié)合 CDR1 CDR2 CDR3 CDR1 CDR2 CDR3 CDR: Complementarity Determining Region FR: Framework Region FR1 CDR1 FR2 CDR2 FR3 CDR3 FR4 H L Complementaritydetermining regions (CDRs): Antigenic Determinant Specificity 特異性 Features of AgAb Reaction Reversibility可逆性 Ag+Ab→Ag 在一定條件下 (如低 pH、高濃度鹽等 )可以解離。Yalow，1960) IRMA(immunoradiovelric assay, Milesamp。Perlmann, van Weemen and Schuurs , 1971) Copyright 169。51:24152418 Estimates of the number of articles published per 5year period from 1960 to 2022 Figure. Estimates of the number of articles published per 5year period from 1960 to 2022. The search was done in February 2022 in PubMed/National Library of Medicine, NIH, with the search terms: enzymeimmunoassay, enzymelinked immunosorbent assay (EIA/ELISA bined), and RIA. (Ordinate), number of articles in which the keywords are quoted. (Abscissa), 5year periods from 1960 to 2022. , bined EIA/ELISA。 the trends, however, are evident.] ?基礎(chǔ) : 抗原或抗體的固相化 抗原或抗體的
教學(xué)課件相關(guān)推薦
鄂ICP備17016276號-1