疫學(xué)常用實驗方法(2)(完整版)
【正文】 ined), and RIA. (Ordinate), number of articles in which the keywords are quoted. (Abscissa), 5year periods from 1960 to 2022. , bined EIA/ELISA。 細(xì)胞核 非生命顆粒 染色體 其它細(xì)胞器以及乳化微球等。 SCID： Severe bined immune deficiency 嚴(yán)重聯(lián)合免疫缺陷小鼠 T、 B淋巴細(xì)胞功能均缺失 A variety of knockout mice: μMT, RAG1/, RAG2/ IFNγ/, IL4R/ … Transgenic mice: GFPtransgenic mice Generation of transgenic mice ?Animal experiments Isolate lymphocytes from spleen or lymph nodes Isolate tumor cells from tumor tissue. Adoptive transfer: T, B, sera… Bone Marrow Chimera ?Advanced animal experiment Skin graft Thank You! Topics ? Immunemediated angiostasis in tumor development. ? MDSC (myeloidderived suppressor cells) in tumor development. ? Th17 cells in tumor development. ? Treg cells in tumor development. ? Fibroblast in tumor development. ? The role of TNF and its receptors in tumor immunity. ? The role of Interferonγ and its receptor in tumor immunity. ? The role of Interleukin 4 and its receptor in tumor immunity. ? Cytokines and inflammation resolution. ? The role of liver fibrosis in development of hepatocellular carcinoma. ? 要求： ? 每組從 topic列表中任選一篇相關(guān)文章（ Journal of Immunology, Nature, Nature Immunology, The Journal of Clinical Investigation, Cancer Research, Science, The Journal of Experimental Medicine, Nature Medicine。 ? 實驗對照的設(shè)計 陰性對照：常用同型抗體對照 陽性對照：單陽性對照（多色分析時用于儀器校正） ? 抗體的選擇 首選直標(biāo)抗體 熒光分子 ? 實驗標(biāo)本的處理 單細(xì)胞懸液的制備 抗體濃度 非特異結(jié)合的去除：洗滌和封閉 4. Western Blott 5. Hybridoma Technique amp。 ELISA ,顯色 ，讀取數(shù)據(jù)。Yalow，1960) IRMA(immunoradiovelric assay, Milesam
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