【正文】
. Key words: 2 , 6dimethyl aniline。 tetramethyl benzidine as new substrates of peroxidase, the enzyme immunoassay (EIA) and enzymelinked immunosorbent test (ELISA) has gained wide application. 3,339。 tetramethyl benzidine has been gradually replaced by strong carcinogens benzidine and other carcinogenic benzidine derivatives, the application in the clinical laboratory, field of forensic examination, forensic and environmental monitoring. Especially in the aspects of clinical and biochemical examination, 3,339。 tetramethylbenzidine is a new kind of safe color reagent. With traditional chromogen reagents such as benzidine, adjacent tolidine and pared ophenylendiamine etc, has the advantages of high detection sensitivity, good stability, and the use of safe, no carcinogenic and mutagenic effects. Currently 3,339。Tetramethylbenzidine HuangLiyan,Grade 2020,Pharmarcettical Engineering Abstract: 3,339。-四甲基聯(lián)苯胺 The Synthesis of 3,339。 關(guān)鍵 詞 : 2,6-二甲基苯胺;溴化;偶聯(lián); 3,339。產(chǎn)品經(jīng) 1HNMR表征。,5,539。 可以作為一種專用于人體神經(jīng)組織酶標(biāo)和免疫酶標(biāo)的研究及血液檢驗(yàn)的試劑。,5,539。四甲基聯(lián)苯胺已在逐步取代強(qiáng)致癌物聯(lián)苯胺和其他致癌 性的聯(lián)苯胺衍生物 ,應(yīng)用于臨床化驗(yàn)、法醫(yī)檢驗(yàn)、刑事偵破及環(huán)境監(jiān)測領(lǐng)域。目前 3,339。,5,539。,5,539。目 錄 第一章 前言 ................................................................................................................................................. 2 3, 3', 5, 5' 四甲基聯(lián)苯胺的概述 .......................................................................................... 2 國內(nèi)外有關(guān) 3, 3', 5, 5' 四甲基聯(lián)苯胺的研 究進(jìn)展 .............................................................. 4 選題意義 .............................................................................................................................................. 5 第二章 實(shí)驗(yàn)部分 ......................................................................................................................................... 6 儀器和試劑 ........................................................................................................................................... 6 實(shí)驗(yàn)儀器 ....................................................................................................................................... 6 實(shí)驗(yàn)試劑 ....................................................................................................................................... 6 3, 3', 5, 5' 四甲基聯(lián)苯胺的合成與研究 .............................................................................. 7 合成原理 ....................................................................................................................................... 7 合成方法 ....................................................................................................................................... 7 第三章 結(jié)果與討論 ..................................................................................................................................10 溴化反應(yīng) ............................................................................................................................................10 偶聯(lián)反應(yīng) ............................