【正文】 光和耐磨性，而且可使織物富 有滑爽和硬挺的外觀。在醫(yī)藥工業(yè)中，由殼聚糖制得的手術(shù)縫合線機(jī)械強(qiáng)度好，可長期存放，能用常規(guī)方法消毒，能被人體內(nèi)組織液降解而吸收，傷口愈合后無須拆除手術(shù)線，免除了患者拆線的痛苦。 殼聚糖與分子印跡技 術(shù) 殼聚糖 及其應(yīng)用 簡介 殼聚糖 (CTs)是天然多糖中唯一的堿性多糖 ， 具有許多獨(dú)特的生物學(xué)功能如：親水性、生物相容性和生物可降解性，其降解產(chǎn)物對(duì)人體健康無害。本文 通過分子印跡技術(shù) 制備出的 殼聚糖分子印跡聚合物 ， 在吸附性能 和 方面有很大程度的提高。 楊超： Pb2+離子印跡殼聚糖微球的制備及識(shí)別性能的研究 2 第 1章 綜述 鉛 (II)是一種無機(jī)環(huán)境激素，在人體內(nèi)聚集到一定程度就會(huì)影響人的正常代謝活動(dòng)，對(duì)人體造成嚴(yán)重危害，而 礦物加工、金屬鍍膜等工廠的工業(yè)廢水中常常含有少量的 鉛 (II)金屬離子 ， 會(huì)對(duì)人體造成一定的危害 。 本文通過分子印跡技術(shù)制備出的殼聚糖分子印跡聚合物，在吸附性能和方面有很大程度的提高。 掌握分子印跡技術(shù)的基本原理及實(shí)驗(yàn)方法。在紡織工業(yè)中，用殼聚糖醋酸溶液作直接染料和硫化染料的固化劑，不僅可以增進(jìn)織物和花布的耐光和耐磨性，而且可使織物富有滑爽和硬挺的外觀。在醫(yī)藥工業(yè)中，由殼聚糖制得的手術(shù)縫合線機(jī)械強(qiáng)度好，可長期存放，能用常規(guī)方法消毒，能被人體內(nèi)組織液降解而吸收，傷口愈合后無須拆除手術(shù) 線，免除了患者拆線的痛苦。 殼聚糖 (CTS)是天然多糖中唯一的堿性多糖， 具有許多獨(dú)特的生物學(xué)功能如：親水性、生物相容性和生物可降解性，其降解產(chǎn)物對(duì)人體健康無害。近十年來， MIPs 在諸如仿生傳感器 [1]、固相萃取 [2]、藥物分析 [3]、色譜分析與分離 [4]、膜分離 [5]、模擬 酶催化 [6]等方面均呈現(xiàn)出良好的應(yīng)用前景，有望在生物工程、臨床醫(yī)學(xué)、天然藥物、食品工業(yè)、環(huán)境監(jiān)測等行業(yè)得到廣泛應(yīng)用。與傳統(tǒng)的分離或分析介質(zhì)相比， MIPs 的突出特點(diǎn)是對(duì)被分離物具有高度的選擇性。 關(guān)鍵詞： 分子印跡，殼聚糖， Pb2+離子 ，吸附等溫線 ， 吸附動(dòng)力學(xué)，印跡效果，選擇性，再生性能 楊超： Pb2+離子印跡殼聚糖微球的制備及識(shí)別性能的研究 II Preparation and Recognition property of iron imprinted chitosan microspheres of Pb2+ Abstract Molecular imprinting technique refers to the preparation of a specific target molecules (template molecules, molecular imprinting or imprinted molecule ) is a specific selective polymer technology, this technology for preparing polymer called molecular imprinting polymer, its outstanding characteristic is to isolate with high selectivity. In addition to molecularly imprinted polymers also has stable physical and chemical properties and mechanical properties, high temperature, high pressure, resistance to acid, alkali, high concentration of ions and anic solvents, and can be used repeatedly. Chitosan is a natural polysaccharide only alkali polysaccharide, the molecules in the presence of amino, hydroxyl and amide, capable of selectively coordination or adsorption of several metal ions, especially for transition metal ions has good chelating ability, and nontoxic, no pollution, in medicine, water treatment and environmental protection etc. the application has been widespread attention. Based on the Pb2+ ion as a template, chitosan as monomer, glutaraldehyde as crosslinking agent, liquid paraffin and Twain 80as oil phase, imprinting and non imprinted chitosan acetic acid solution as the water phase, using a reverse emulsion preparation of non imprinted chitosan and Pb2+ Imprinting Chitosan Microsphere polymer. Studying and paring the two adsorption isotherms and adsorption kiics, as well as Pb2+ Imprinting Chitosan Microspheres polymer imprinting effect, selective and regeneration performance. The results showed that: ① the ERN and Pb2+ Imprinting Chitosan Microspheres adsorbing Pb2+ ions are consistent with the Langmuir adsorption isotherm. ② Pb2+ Imprinting Chitosan Microspheres polymer recognition template Pb2+ ion, at a concentration of100mg/L, imprinting factorα value maximum, said this manifestation of imprinting effect best. At the temperature of 40℃ , pH 3, non imprinted chitosan microsphere adsorption capacity , Pb 2+ Imprinting Chitosan Microspheres for saturated adsorption capacity , the saturated adsorption capacity close to two times that of the former, and strong adsorption capacity. ③ non imprinted chitosan microspheres and Pb2+ Imprinting Chitosan Microspheres adsorbing Pb2+ ions are consistent with a firstorder kiic adsorption equation. ④ Pb2+ Imprinting 安徽工程大學(xué)畢業(yè)論文 III Chitosan Microspheres polymer template Pb2+ ion has a specific selectivity, at low concentrations were higher, with the concentration increased and decreased gradually. The Pb2+ imprinting chitosan microsphere adsorption performance and stability in the three cycle, fourth times the equilibrium adsorption capacity slightly decreased number of. And, at least it can be recycled7~8. Key words: molecular imprinting, chitosan, Pb2+ ion, adsorption isotherms, adsorption kiics, imprinting effect, selective, regeneration performance. 楊超： Pb2+離子印跡殼聚糖微球的制備及識(shí)別性能的研究 IV 目 錄 引言 ............................................................................................................................... 1 第 1 章 綜述 ....................................................................................................................... 2 殼聚糖與分子印跡技術(shù) .............................................................................................. 2 殼聚糖及其應(yīng)用簡介 ............................................................................................ 2 分子印跡技術(shù)的基本原理和發(fā)展概況 ................................................................ 2 殼聚糖分子印跡聚合物的國內(nèi)外研究現(xiàn)狀 .............................................................. 4 對(duì)金屬離子吸附性能的研究 ................................................................................ 4 對(duì)小分子有機(jī)物吸附性能的研究 ........................................................................ 5 對(duì)蛋白質(zhì)分子吸附性能的研究 ............................................................................ 5 課題研究目的意義 ...................................................................................................... 6 課題研究主要內(nèi)容 ...................................................................................................... 6 第 2 章 實(shí)驗(yàn)材料和實(shí)驗(yàn)方法 ........................................................................................... 7 實(shí)驗(yàn)材料 ...................................................................................................................... 7 實(shí)驗(yàn)藥品 ................................................................................................................ 7 實(shí)驗(yàn)器材 ................................................................................................................ 7 殼聚糖 聚合物的制備 .................................................................................................. 7 非印跡殼聚糖微球的制備 ...................................................................................