國外食品檢驗(yàn)培訓(xùn)(ppt28)(英文)-質(zhì)量檢驗(yàn)-文庫吧資料
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【正文】 methods and rapid methods Bureau for International Research and Technology Cooperation Austria Conventional Methods ? Conventional methods rely on culturing microansims onto agar plates ? Laborious and timeconsuming ? 84% of all tests arebased on counting viable cells ? Required by national and international regulatory agencies for official control Bureau for International Research and Technology Cooperation Austria Rapid Methods ? Advantages in analysis time ? Possibility to eliminate labour intensive steps ? Potential for automation ? However, lengthy incubation procedures are still necessary ? First oneshift methods under development ? ELISA and PCR are dominating ? Lack of a mon validation procedure ? Mostly not accepted for official control purposes Bureau for International Research and Technology Cooperation Austria Methods ? Advances in Viable Cell Count Methods ? Miniaturisation and Diagnostic Kits, Biochemical Identification Techniques ? Antibodybased methods ? Immunomagic Separation ? Nucleic acidbased assays ? Biosensors ? Microarrays ? Flow Cytometry ? BacteriophageBased Techniques ? ATP bioluminescence ? Adenylate kinase bioluminescence ? Riboprinting and PulseField Gel Electrophoresis Bureau for International Research and Technology Cooperation Austria Viable cell counting ? Will remain important for the assessment of safety and quality of food products ? development of semiautomated alternative methods ? new methods are based on growth and metabolic activity ? Microscopic methods ? flow cytometry and cell sorting (FACS) Bureau for International Research and Technology Cooperation Austria Immunoassays ? ELISA (with dipstick technology), Immunochromatography ? Fully automated systems already on the market ? New rebinant antibodies and molecular imprinting techniques to improve sensitivity and versatility Bureau for International Research and Technology Cooperation Austria Immunomagic Separation
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