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分子分型在醫(yī)院感染控制中的作用(參考版)

2025-06-15 18:13本頁面
  

【正文】 ? 由此可見,分子流行病學(xué)方法: ? 為醫(yī)院感染控制 提供準確的實驗數(shù)據(jù) ? 有效判斷感染來源和流行趨勢 ? 為更好的做好醫(yī)院感染控制工作打下了基礎(chǔ)。 ? 14 strains: from one of two clones (88%) ? Indicating: a high probability of nosoial spread ? Review: ? microbiology laboratory: culture requisitionsno close contact. ? Patients: existing direct connection between 11/14 patients (14). ? infection control practices: aborted the outbreak ? 典型的醫(yī)院感染傳播 ? VRE: 19株 , 來自 16個病人, 2個月時間內(nèi) 。 ? nosoial outbreak。 ? any needed changes were determined. ? The anizational structure for selecting microbes for typing was shared by the medical directors of infection control and clinical microbiology 29 需基因分型的微生物: ? routinely genomically typed: VRE ? Periodic routine typing: ? fluoroquinoloneresistant P. aeruginosa, ? methicillinresistant Staphylococcus aureus (MRSA), ? Enterobacter cloacae, ? Clostridium difficile ? Additional anisms for typing:selected through surveillance of microbiology culture reports discussed at the weekly meeting. ? 常規(guī)基因分型: VRE ? 周期性分型: ? 氟喹諾酮耐藥的銅綠 ? MRSA ? 陰溝腸桿菌 ? 難辨梭菌 ? 其他:根據(jù)微生物報告并在周會上討論后決定需基因分型的病原體 任何時候 工作小組要求進行基因分型,臨床微生物實驗室即將菌株提供給 分子分型部門 并執(zhí)行 30 實驗方法 REA analysis restriction of genomic DNA with conventional electrophoresis DNA限制性酶切 31 基因分型相關(guān)費用 ? the cost of equipment, remodeling, ? reagent and other supplies, ? salaries and benefits for three technologists, ? plus all the institutional assessments (., fullcost basis) required to operate a hospital laboratory. ? 儀器配制及維修、實驗室改造等; ? 試劑及其他消耗品 ? 三位實驗員的薪水 ? 所有的評估分析(如fullcost basis )需要動用全院系統(tǒng) 分析方法: t檢驗 成本 效益分析( Analysis of Cost Data) 32 結(jié)果: ? VRE ? initial impetus: serious nosoial problemVRE39。 medical records, ? direct observation of medical and nursing practice, ? active surveillance of rectal cultures of patients in nursing units for highrisk patients, ? evaluation of suspected nosoial infections reported by healthcare providers. ? 查閱微生物報告 ? 查閱病史記錄 ? 對醫(yī)護人員操作的直接觀察 ? 高危病人直腸培養(yǎng)的動態(tài)連續(xù)觀察 ? 對上報的可疑醫(yī)院感染的評估鑒定 由三位專職感染控制人員對資料進行匯總分析,并制定出相應(yīng)控制措施,并在醫(yī)院感染控制和預(yù)防部門主管的指導(dǎo)下實施。34(5):1129~ 1135 20 分子分型在醫(yī)院感染控制中的作用 21 Special Issue New Technology for Detecting MultidrugResistant Pathogens in the Clinical Microbiology Laboratory Lance R. Peterson*? and Gary A. Noskin*? *Northwestern Memorial Hospital and ?Northwestern University Medical School, Chicago, Illinois, USA EID, 2022, 7: 306 22 ? Northwestern Memorial Hospital, Chicago ? 700bed, universityaffiliated medical center ? 出院: 39,000/年 ? 急診: 56,000例 /年
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