mir396在豆科植物苜蓿根中對(duì)其菌根化和分生組織活性的影響(參考版)
2025-05-10 18:10本頁(yè)面
【正文】 1, histone H4, CDC16) or endocycle (CCS52B) markers was measured using quantitative RTPCR in miR396OE and control roots The percentage of cells replicating (. EdUpositive nuclei) obtained from 20 root tips per construct in four independent experiments ? Together, these data strongly suggest that miR396dependent modulation of root growth may essentially be due to a restriction of cell division activity. 2022/6/4 23 ? Silencing of MtGRF genes mimics the miR396OE root growth phenotype ? To study the role of the miR396 targets on root growth, we inactivated them using an RNAi strategy. ? First, we generated posite plants expressing three RNAi constructs targeting a conserved region of various GRF genes ( MtGRF2, MtGRF4 and MtGRF6). ? In addition, we prepared an RNAi vector targeting both MtbHLH79 genes and confirmed their efficient silencing 2022/6/4 24 2022/6/4 25 Silencing of MtGRF genes mimics miR396 overexpression in roots. iGRF表達(dá)減少而 ibHLH79表達(dá)不變，說(shuō)明很可能是miR396對(duì)GRF基因的負(fù)調(diào)控使得miR396OE和MIM396植株中根的生長(zhǎng)表型不同 ? Hormonal control of miR396 expression ? We treated wildtype plants for 1, 3 or 5 h with auxin, cytokinin, gibberellin, abscisic acid or brassinosteroids (BR), and measured premiR396 and target mRNA levels in roots. 2022/6/4 26 2022/6/4 27 Gibberellin, cytokinin and auxin treatments did not affect expression of any of these genes 2022/6/4 28 miR396b對(duì) ABA和 BR 有明顯的反應(yīng)，并且GRF5在 BR處理下下調(diào)，說(shuō)明BR和 miR396b, GRF5之間有某種作用關(guān)系 2022/6/4 29 Levels of GUS transcript in roots expressing the prommiR396b:GUS construct (grey bars) or the promGRF5:GUS construct
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鄂ICP備17016276號(hào)-1