疾病蛋白質(zhì)組學(xué)ppt課件(參考版)
2025-01-08 13:52本頁面
【正文】 5:4443–4455) ? cytoskeleton and cytoskeletonbinding proteins (tubulin, actin, cofilin, vimentin, etc.) ? membraneassociated proteins that control transport and ? signalling (caveolin, annexins, dynein, etc.) ? foldingchaperones (calnexin, calreticulin, etc.) ? Adhesion molecules, such as ICAM1 and integrins b1, a5 and a2 The role of Exosomes ? modulate immune response ? regulate haemostatic balance – support thrombin generation and induce expression and secretion of plasminogen activator inhibitor1 by endothelial cells – attenuating fibrinolysis and promoting prothrombotic conditions ? ability to be absorbed to the cell surface and mediate cellcell interactions in the cardiovascular system Proteomics of exosomes ? dendritic cellderived exosomes (J. Immunol. 2022, 166, 7309–7318.) – endocytic proteins were abundant ponents of the proteome of exosomes. – 21 new exosomal proteins were identified, including cytoskeletonrelated proteins, such as cofilin, profilin I or elongation factor 1a, and intracellular membrane transport proteins, such as annexins, rab7, 11, rap 1B, and syntenin. – a series of apoptosisrelated proteins, including thioredoxin peroxidase II, Alix, 1433, and galectin3. ? mastcell derived exosomes (Arterioscler. . Biol. 2022, 25, 1744–1749) – regulate the secretion of plasminogen activator inhibitor1 by endothelial cells possibly by ? prothrombinase plex ? TNFa ? angiotensinogen precursors. 5. Proteomics of the secretome ? “secretome” referred to the plex collection of proteins secreted by a particular type of cell often maintained in vitro. ? the analysis of the secretome of different blood and vascular cell types could be of critical importance in the clarification of heterogeneous cellcell interactions and their regulation by autocrine and paracrine factors. ? The limited plexity of the secretome makes it suitable for the application of a proteomic approach Challenges to Proteomics of the secretome ? It is difficult to pletely avoid crosscontamination with proteins of the serum supplement monly used in cell cultures, although the conditioned medium is usually sampled after extensive washing and during incubation in serumfree medium. ? Any variation in the carryover of serum proteins has a profound impact on quantitative parisons ? Cell death and cytoplasmic protein release in the culture medium is a source of false positives, which further impairs the reliability of proteomic analysis of the secretome. Technical innovations to improve the capability of secretome analysis ? proteinenrichment by precipitation (. carrierassisted TCA precipitation) [Proteomics 2022, 7: 1757–1770] ? highabundance serum protein depletion (. sodium chloride/ethanol precipitation) [Proteomics 2022, 5: 2656–2664]), ? LC fraction (. RP tC2 Sorbent) [J. Proteome Res. 2022, 5: 899–906]) ? dialysis/ultrafiltration methods [J. Microbiol. Methods 2022, 68: 396–402]. SUMMARY 一、疾病蛋白質(zhì)組學(xué)（ disease proteomics）概念和總體研究概況 二、心血管疾病蛋白質(zhì)組學(xué) 1. The myofilament proteome. 2. Redox modifications in the cardiac proteome. 3. Cardiac biomarkers. 4. Secretory microvesicles 5. Proteomics of the secretome 。 – physically mediate leukocyteleukocyte and leukocyteendothelium interactions via direct binding of cell surface receptors Proteomics of microparticles Proteomic analysis of protein expression in human plasma microparticles. Microparticles derived from the peripheral blood by centrifugation were lysed and labelled with Cydyes (green and red colour in A and B, respectively). Using DIGE, microparticle and microparticledepleted plasma proteins were coseparated in large format 2D gels. Images were acquired
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