微生物培養(yǎng)基culturalmedium-資料下載頁(yè)
2025-01-01 00:52本頁(yè)面
【正文】 improvement of cell permeability 滲透性 protection on enzyme activity 培養(yǎng)基的設(shè)計(jì)及優(yōu)化 media design and its optimization ? Importance of media design ? Current situations of media design: many factors ? empirical 經(jīng)驗(yàn) + scientific ? 培養(yǎng)基成分選擇的原則 (1) 菌體的同化能力： large molecules + enzymes ? small molecules ? Selection of carbon sources: glucose, starches 不同糖化工藝所得糖液質(zhì)量的比較 項(xiàng)目 酸法 酸酶法 雙酶法 葡萄糖值 (DE值 ) 葡萄糖含量％ 灰分％ 色度 羥甲基糠氨％ 葡萄糖收率 91 86 95 93 較酸法高 5％ 98 97 較酸法高 10％ ? Selection of nitrogen sources: If the microbe produces proteolytic enzymes, a variety of crude nitrogen sources such as soybean meal can be used. If the microbe lack of proteases, the anic nitrogen source should be hydrolyzed. 豆餅粉＋ water + hydrochloric acid 鹽酸 , 100176。 C ? amino acids. ? (2) 代謝物的阻遏和誘導(dǎo) 葡萄糖效應(yīng) bination of anic and inanic nitrogen/carbon sources enzyme agent production: For many induced enzymes, starch and dextrin are mon carbon source. 碳源對(duì)生長(zhǎng)和產(chǎn)酶的影響 碳源 地衣芽孢桿菌 細(xì)胞量 g/l 淀粉酶活 /(U/ml) glucose 0 Fructose Sucrose Dextrin starch 0 0 age of microanisms inducement and inhibition of nitrogen source for some products. (3) 合適的 C、 N比 The ratio of C、 N have greatly effects on microbial growth and product synthesis 一般工業(yè)發(fā)酵培養(yǎng)基的 C、 N比為 100: (~) Many factors influence the optimal ratio (4) pH的要求 ? A important environment factor ? Fluctuation (波動(dòng) ) of cultural pH ? Optimal media formulation is the key factor to avoid fluctuation of cultural pH ? 除考慮營(yíng)養(yǎng)需求，也要考慮其代謝后對(duì)培養(yǎng)體系 pH緩沖的貢獻(xiàn)，使 pH 處于較適宜的狀態(tài) 培養(yǎng)基的優(yōu)化 media optimization ? The best media formulation is decided by tests. ? A general procedure of media optimization: (1) 根據(jù)前人的經(jīng)驗(yàn)和培養(yǎng)基成分選擇的原則，初步確定可能的培養(yǎng)基成分； (2) decision of best media with single factor test (3) optimization of each ponent concentration by some test design. The criterion for a best industrial medium ? 1. 培養(yǎng)基能夠滿足產(chǎn)物的最經(jīng)濟(jì)合成 ? 2. Byproduct concentration as low as possible ? 3. The raw materials are cheaper, easy to transport and store. The supply is steady. ? 4. 能滿足總體工藝的要求，如不影響通氣、提取、純化及廢物處理 謝謝觀看 /歡迎下載 BY FAITH I MEAN A VISION OF GOOD ONE CHERISHES AND THE ENTHUSIASM THAT PUSHES ONE TO SEEK ITS FULFILLMENT REGARDLESS OF OBSTACLES. BY FAITH I BY FAIT
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