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【正文】 d directly to boiling Laemmli buffer. Duplicate samples will be transferred to a nitrocellulose membrane, and the radiolabeled proteins will be visualized with a PhosphorImager. The same membranes then will be immunoblotted with either the antiSun1 serum or the antiV5 antibody. 5. Reconstitute Rebinant Protein Fragment Functionally into Detergent Micelles In order to prepare samples for NMR study, we will first choose suitable detergent micelles to reconstitute the rebinant protein fragment , use a direct dilution method to refold the proteins, and test the refolding of the target protein by radioligand binding. 6. NMR and Xray Crystallography Studies We will examine whether solution NMR studies of the micelle systems ( using different domains of the target protein ) will give sufficient information about the structures of human Sun1. As for Xray crystallography studies of the whole protein , we will choose a suitable detergent first to work out protein crystals and then perform structural studies on the crystals . FURTHER STUDIES ? With the aid of NMR and Xray crystallography, we can study directly proteindrug interactions and proteinprotein interactions. ? We will predict human Sun 139。 s 3D structure by molecular modeling and pare it with experimental data from NMR or Xray crystallography. 1. 若不給自己設(shè)限，則人生中就沒(méi)有限制你發(fā)揮的藩籬。2. 若不是心寬似海，哪有人生風(fēng)平浪靜。在紛雜的塵世里，為自己留下一片純靜的心靈空間，不管是潮起潮落，也不管是陰晴圓缺，你都可以免去浮躁，義無(wú)反顧，勇往直前，輕松自如地走好人生路上的每一步3. 花一些時(shí)間，總會(huì)看清一些事。用一些事情，總會(huì)看清一些人。有時(shí)候覺(jué)得自己像個(gè)神經(jīng)病。既糾結(jié)了自己，又打擾了別人。努力過(guò)后，才知道許多事情，堅(jiān)持堅(jiān)持，就過(guò)來(lái)了。4. 歲月是無(wú)情的，假如你丟給它的是一片空白，它還給你的也是一片空白。歲月是有情的，假如你奉獻(xiàn)給她的是一些色彩，它奉獻(xiàn)給你的也是一些色彩。你必須努力，當(dāng)有一天驀然回首時(shí)，你的回憶里才會(huì)多一些色彩斑斕，少一些蒼白無(wú)力。只有你自己才能把歲月描畫(huà)成一幅難以忘懷的人生畫(huà)卷。
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