研修計(jì)劃要點(diǎn)(英文)(更新版)
【正文】 l status: I am *****, female, born on 24th of Dec., 19**. My parents are very successful in their careers. My father is **** and my mother is ****. Through parison, I finally choose to study in the Canada because of her famous education, beautiful scene and safe living place.The Reason for Choosing *****(你要去的學(xué)校的介紹):The Proposed Courses: And I’d like to share every step of success with my dear parents.Respectful officer, if I am luck to get the permission to go to Canada to further my study, which I believe is the first step to approach my goal, I will treasure this opportunity, make every effort to improve myself. s orientation and in vivo interactions with other molecules. Next we will purify the rebinant protein by affinity chromatography which is one of the two suitable methods to study membrane proteins. After the purification, we will refold the protein with the aid of micelles or liposomes in order to prepare samples for NMR study. S tudies have shown that Sun1 is detergent, salt, and chaotrope resistant . Fortunately, it is also shown that treatment with detergent and medium salt concentrations (1% Triton X100/ 200 mM NaCl) can efficiently solubilize Sun1, which can be a good start for our study. Currently, membrane proteins can be studied either by NMR or Xray crystallography. However, for NMR, only protein fragments with molecular weight less than 20 kDa can be studied. For the limitation of NMR, we can study independently the transmembrane domains of Sun1 by NMR, and bine the experimental data, which can sometimes give sufficient information about the target protein. EXPERIMENTAL PROCEDURE ?用一些事情，總會(huì)看清一些人。
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