浙江大學(xué)膠體金免疫標(biāo)記培訓(xùn)班資料-資料下載頁(yè)
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【正文】 rown and root rot in tomato plants. Phytopathology 84: 1432–14444. Brangeon J and Sossutzov L. l993. Electron microscopic in situ hybridization to RNA and DNA in plant cells. in Morel G ed., Hybridization Techniques for Electron Microscopy. Boca Ratront: CRC Press. pp3023485. Couble P. 1993. Principles of probe preparation for in situ hybridization. In: Morel G ed., Hybridization Techniques for Electron Microscopy. Boca Ratront: CRC Press. pp:45636. Frens G. 1973. Controlled nucleation for the regulation of the particle size in monodisperse gold in the demonstration of cell surface markers. Histochemistry, 72:83907. Hodges GM, Smolira MA and Livingston DC. 1984. Scanning electron microscope immunocytochemistry in practice. in Polak JM and Varndell IM ed., Immunolabelling for Electron Microscopy. Elsevier Science Publishers B. V. pp:l902338. Horisberger M and Rosset J. 1977. Colloidal gold, a useful marker for transmission and scanning electron microscopy. The J Histochem. and Cytochemi., 25(4):2953059. Hyatt AD and Eaton BT ed, Immunogold electron microscopy in virus diagnosis and research. Boca Raton: CRC Press. 199310. McFadden GI. 199l. In situ hybridization techniques: Molecular cytology goes ultrastructural. in。 Hall JL ed., Electron Microscopy of Plant Cells. London: Academic Press. pp:2l925511. PuvionDutilleul F. l993. Procedures of in situ nucleic acid hybridization to detect viral DNA and RNA in cells by electron microscopy. in Morel G ed., Hybridization Techniques for Electron Microscopy. Boca Ratront: CRC Press. pp26929912. Roth J. l984. The protein Agold technique for antigen localization in tissue sections by light and electron microscopy. in Polak JM and Varndell IM ed., Immunolabelling for Electron Microscopy. Elsevier Science Publishers B. V. pp:1l312l13. Slot JW and Genze HJ. 1985. A new method of preparing gold probes for multiplelabelling cytochemistry. Eur J Cell Biol. 38:879114. Slot JW and Geuze HJ. l984. Gold markers for single and double immunolabelling of ultrathin cryosection. In Polak JM and Varndell ed., Immunolabelling for Electron Microscopy. Elsevier Science Publishers B. V. pp: l29l4215. Vandenbosch KA. l99l. Immunogold labelling. In: Hall JL ed., Electron microscopy of plant cells. London: Academic Press. pp:1812l8常見(jiàn)膠體金探針制備條件（10ml直徑10nm膠體金）探針蛋白來(lái)源分子量(kD)交聯(lián)pH最佳標(biāo)記pH蛋白用量(mg/ml)重懸浮液特異性標(biāo)記底物IgG哺乳動(dòng)物150160120BL1各種抗原IgG FabIgG水解45150BL各種抗原Protein A葡萄球菌42/32(reb)6060BL多種抗體(Fc片段)Protein G鏈霉菌454790BL多種抗體(Fc片段)麥胚凝集素(WGA)大麥36125BL幾丁質(zhì)伴刀豆凝集素(Con A)伴刀豆1022501mM Ca2++1mM Mn2+ in BLa甘露糖a葡萄糖幾丁質(zhì)蓖麻凝集素I(RCA I)蓖麻12060BLb半乳糖a半乳糖幾丁質(zhì)大豆凝集素(SA)大豆11065BLD氨?；肴樘腔ㄉ?PA)花生110130BLD氨酰化半乳糖親和素(avidin)雞蛋67120BL生物素(biotin)鏈親和素(streptavidin)鏈霉菌60200BL生物素(biotin)b1,3葡聚糖酶煙草180BLb1,3葡聚糖纖維素酶綠色木霉160BL纖維素漆酶(lacasase)白色腐霉100BL (pH )多酚類(lèi)化合物通用BL配方：50 mM PBS, pH ,，內(nèi)含1% BSA，% PEG20000，100 mM NaCl，1% NaN3。
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