分光光度法測(cè)定多巴胺王懷友(精)-資料下載頁(yè)
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【正文】 社 , 1986. 122Spectrophotometric determination of dopamine W ANG Huai you , SU N Yue and TANG Bo (Department of Chemistry, Shangdong Normal University, Jinan 250014 , Fenxi Shiyanshi, 2003, 22(1 :45~47 Abstract :A spectrophotometric method was developed for the determination of dopamine. The method was based on the reaction of dopamine with sodium nitrite in aqueous medium, and the product had a maximum ab sorption at 300nm, with E 300=1. 85@104l mol 1 cm 1. Effec ts of pH, heating time, standing time and foreign ions were tested. The absorbance for dopamine from 0to 10L g P mL obeyed Beer c s law. The linear re gression equation of the calibration graph was A = 010436c +0100591, with a correlation coefficient of 019978. The detection limit of this method was 0. 1L g P mL and recovery was from 93. 3%to 110. 5%.The method was used for the deter mination of dopamine in injec tion solution of dopa mine hydrochloride.Keywords :Spec trophote metry。 Dopa mine。 Sodium ni trite。 Injectio
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