【正文】 the abalone Haliotis diversicolor aquatilis seeds. The death rate is to be reduced by adopting such prevention and treatment skills as protecting the environmental ecology, adding nutrition to the bait, providing medical and physical treatment, etc. The average breeding density of the abalone haliotis diversicolor aquatilis seeds is over 1000 per .聯(lián)系單位：廈門集美大學聯(lián)系人：蘇國成電 話：05926180464 傳 真：05926181263 2空腸彎曲菌的磁捕獲熒光PCR檢測新技術New Technology of Magnetic CaptureFluorescent PCR Detection of Campylobacter jejuni應用抗血清和磁性微珠自行制備彎曲菌免疫磁珠，利用彎曲菌免疫磁珠直接捕獲檢樣中目的菌，不需要增菌培養(yǎng)；設計用于同時檢測空腸彎曲菌鞭毛蛋白A（flaA）基因和馬尿酸酶（hipO）基因的引物與熒光標記探針，通過熒光PCR檢測flaA基因和/或hipO基因，建立空腸彎曲菌的磁捕獲熒光PCR檢測新技術；該技術方法簡便易行，可在一個工作日內完成，特異性好，敏感度高，抗干擾性強，可以鑒別區(qū)分空腸彎曲菌與其它彎曲菌，檢測低限達到10cfu/mL，解決了活的非可培養(yǎng)狀態(tài)或受損傷的空腸彎曲菌檢測難題；該技術方法申請了發(fā)明專利（），適用于檢驗檢疫、疾病預防和農(nóng)產(chǎn)品安全檢驗等領域的快速檢測。In order to develop a rapid method which can check Campylobacter jejuni in animal and poultry foods nicely, an immunomagnetic capturefluorescent PCR (IMC FPCR) method was established. The method involves isolation of the target pathogen by immunocapture prior to the fluorescent PCR step, therefore the immunomagnetic beads for Campylobacter were developed, and two groups of primer/ probe, which targeted for the species special sequence of flaA gene and hipO gene for Campylobacter jejuni were designed. The immunomagnetic capturefluorescent PCR assay amplification of the hipO gene and flaA gene for detection of Campylobacter jejuni was firstly reported in this study. Result indicated that IMCFPCR method permits direct detection of the pathogen without an enrichment step and can be performed in approximately 24 h. The assay results are positive for all of the isolates of Campylobacter jejuni (3 isolates, including type strain ATCC 33560 and ATCC8341) with a detection limit of approximately 10 cfu / mL, are negative for Campylobacter coli and several other bacteria. IMCFPCR assay provide not only a rapid, sensitive method for quantitative detection of Campylobacter jejuni, but also an important method for detecting of Campylobacter jejuni of viable but nonculturerable (VBNC) state. The method has been applied for a patent (), and it is suitable for the field of inspection and quarantine, disease control and prevention, agricultural products quality and security.聯(lián)系單位：廈門集美大學聯(lián)系人：劉光明電 話：（0592）6180464 傳 真：（0592）6181263 2龍蝦養(yǎng)殖技術The aquicultural technology of spiny lobster Panalirus stimpsoni龍蝦(the spiny lobster)是大型名貴蝦類，市場需求量巨大，龍蝦商品價格歷來居高不下，人工養(yǎng)殖龍蝦效益可觀，前景看好。本研究成果達到國內領先水平。在陸上水池高密度養(yǎng)殖龍蝦，種苗放養(yǎng)密度可達10尾/m2以上，養(yǎng)殖成活率可達90%，每蝦年增重量高于300g，生產(chǎn)效能高于其它養(yǎng)殖方式。The giant spiny lobster , Panalirus stimpsoni , is a rare marine prawn species. There is a vast foreground in developing an aquicultural project of rearing spiny lobster owing to the great demand in food market as well as its perennial high prices. With a series of doable techniques, the lobsters is to be reared in some seaboard concrete ponds and the number reaches 10 per square meter, the average animal survival rate is high up to 90% and the body weighs over 300g after in one year rearing. All facts bearing out the rearing efficiency applied to aquicultural pattern surpasses the other ways. 聯(lián)系單位：廈門集美大學聯(lián)系人：劉光明電 話：05926180464 傳 真：05926181263 2壇紫菜新品種養(yǎng)殖技術Culture technique of new variety of Porphyra haitanensis本項目利用人工誘變和體細胞單克隆技術等高新技術和傳統(tǒng)的選擇育種相結合的方法，分離出生長快、優(yōu)質，高產(chǎn)，抗高溫等的優(yōu)良品種,在生產(chǎn)上大面積推廣應用，并獲得了分離壇紫菜穩(wěn)定色素突變體的技術和建立壇紫菜的快速良種選育實用技術；新品種畝產(chǎn)提高26%30%，3種主要色素（藻紅蛋白、藻藍蛋白、葉綠素a）提高10%以上，抗病爛能力較強。This program is to explore the high tech and traditional breeding of a new variety of Porphyra haitanensis, in hope of its fast growth, high quality and endurance of high temperature. This variety has been growing various areas, and it shows that is yield increases above to　26 %30%, and the content of three main pigments (phycoerythrin, phycocyanin and chlorophyll a) increases 10 % at least. And it has stronger capability of resisting disease and fester.聯(lián)系單位：廈門集美大學聯(lián)系人：陳昌生電 話：05926180464 傳 真：05926181263 無公害作物生長促劑——海植寶產(chǎn)品開發(fā)The Development of Haizhibao One Plant GrowPromoting Green Product 本研究項目從海帶中提取、分離、濃縮、調配、包裝，制成濃縮液體產(chǎn)品。產(chǎn)品具有作物生長促進劑的作用，能夠顯著提高花果作物的產(chǎn)量，還具有一定的防蟲抗病作用，減少殺蟲劑的使用次數(shù)，提高農(nóng)產(chǎn)品的安全性。The product is in form of liquid made from kelp by extraction, separation, blending and packing. Its main function is to promote crops’ growing, fruit production as well as to keep away worms and to cure diseases. The pesticide could be reduced to minimum amount, which ensure the safety of agricultural products.聯(lián)系單位：廈門集美大學聯(lián)系人：陳昌生電 話：05926180464 傳 真：05926181263 3蝦青素發(fā)酵法生產(chǎn)技術研究Techniques of production astaxanthin by using fermentation method本成果利用發(fā)酵法生產(chǎn)天然蝦青素，對蝦青素產(chǎn)生菌株的分離、高產(chǎn)菌株的選育、蝦青素發(fā)酵工藝的優(yōu)選、蝦青素提取技術的優(yōu)化等進行系統(tǒng)地研究。分離選育出蝦青素高產(chǎn)的海洋紅酵母，在200L中試罐中發(fā)酵蝦青素，蝦青素的產(chǎn)率達6075mg/L發(fā)酵液；對天然蝦青素的無毒提取技術進行研究， mg/g干菌體；蝦青素發(fā)酵水平和提取技術水平為國際先進，國內領先，為用發(fā)酵法生產(chǎn)天然蝦青素打下堅實的基礎。This achievement is mainly about producing natural astaxanthin by fermentation method. Techniques including strains isolation, breeding astaxanthin overproducing strains, optimization of fermentation process and astaxanthin extract process. An astaxanthin overproducing strain was obtained by mutagenesis. In 200L mon stirring fomenter, 60mg/L 70mg/L astaxanthin were harvested by fedbatch cultivation of Phaffia rhodozyma. Results of optimization of extract condition indicated astaxanthin productivity was (dry cell) by acid methods under optimal condition. This achievement reached to the leading level of the field in China, and also advanced method internationally. In the same time, it is a good basis for the further research for the fermentation of natural astaxanthin.聯(lián)系單位：廈門集美大學聯(lián)系人：蔡慧農(nóng)電 話：05926180464 傳 真：05926181263 3三倍體九孔鮑養(yǎng)殖技術The Culturing Technigues of Triploidy abalone Haliotis diversicolor aquatilis本項目利用細胞工程的染色體組操作技術，培育出個體大，生長快，育性低，抗逆性強等優(yōu)點的三倍體九孔鮑，并在生產(chǎn)上應用。在同等養(yǎng)殖條件下，三倍體九孔鮑的殼長和殼重比二倍體九孔鮑分別增長10%和20%，顯示出三倍體顯著的生長優(yōu)勢。 This research manage chromosom