彗星實驗(英語版)-資料下載頁
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【正文】 t as a function of position in the cell cycle.Mut Res,1993,294:275 13Popanda O, Ebbeler R, Twardena D, et al.Radiationinduced DNA damage and repair in lymphocytes from breast cancer patients and their correlation with acute skin,reaction to radiotherapy.Int J Radiat Oncol Biol Phys, 2003, 1255(5): 1216 14Popanda O, Ebbeler R, Twardena D, et al.Radiationinduced DNA damage and repair in lymphocytes of breast cancer patients in parison with acute skin reactions after radiation therapy.Environmental and Molecular Mutagenesis, 2002, 39(Suppl): 50 15Oppitz U, Denzinger S, Nachtrab U, et al.Radiationinduced etformation in human skin fibroblasts from radiotherapy patients with different normal tissue reactions.Strahlenth amp。 Oncol, 1999,175(7): 341 16Djuzenova CS, Rothfuss A, Oppitz U, et al.Respons to Xirradiation of Fanconi anemia (FA) homozygous and heterozygous Qlls assessed by the single cell gel electrophoresis.Laboratory Investigation,2001, 81(2):105DNA鈉鹽的紫外吸收在260nm附近有最大吸收值，在230nm處為吸收低谷，其吸光率（absorbance）以A260表示，A260是核酸的重要性質(zhì)，RNA鈉鹽的吸收曲線與DNA無明顯區(qū)別，蛋白質(zhì)在280nm處有最大的吸收峰，鹽和小分子則集中在230nm處，對于純的核酸溶液，測定A260，即可利用核酸的比吸光系數(shù)計算溶液中核酸的量，核酸的比吸光系數(shù)是指濃度為1μg／mL的核酸水溶液在260nm處的吸光率。據(jù)朗波－比爾光吸收定律A＝lgT＝εbc知，c＝A/εb，而b通常為1cm，所以，通常以1OD值相當(dāng)于50μg／mL雙螺旋DNA，或40μg／mL單螺旋DNA（或RNA），或20μg／mL寡核苷酸計算。Li AF，Sato T，Haimovici R，et al． High glucose alters connexin 43 expression and gap unction intercellular munication activity in retinal pericytesHockemeyer D，Sfeir A J，Shay J W，et al． POT1 protects telomeres from a transient DNA damage response and determines how human chromosomes end
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